PDBsum entry 4x3e

Transcription

PDB id: 4x3e

Contents

Protein chain

359 a.a.

Ligands

ALA-GLN-ARG-M3L-PHE-ALA-GLN-SER

Waters ×175

PDB id:

4x3e

Name:

Transcription

Title:

Crystal structure of eed in complex with a trimethylated jarid2 peptide

Structure:

Polycomb protein eed. Chain: a. Fragment: unp residues 77-441. Synonym: heed,wd protein associating with integrin cytoplasmic tails 1,wait-1. Engineered: yes. Ala-gln-arg-m3l-phe-ala-gln-ser. Chain: b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: eed. Expressed in: escherichia coli. Expression_system_taxid: 562. Expressed in: synthetic construct. Expression_system_taxid: 32630

Resolution:

2.30Å R-factor: 0.174 R-free: 0.224

Authors:

N.Justin,S.J.Gamblin,R.Margueron

Key ref:

S.Sanulli et al. (2015). Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation. Mol Cell, 57, 769-783. PubMed id: 25620564 DOI: 10.1016/j.molcel.2014.12.020

Date:

28-Nov-14 Release date: 21-Jan-15

Protein chain

O75530  (EED_HUMAN) -  Polycomb protein EED from Homo sapiens

Seq: 441 a.a.

Struc: 359 a.a.

DOI no: 10.1016/j.molcel.2014.12.020

Mol Cell 57:769-783 (2015)

PubMed id: 25620564

Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation.

S.Sanulli, N.Justin, A.Teissandier, K.Ancelin, M.Portoso, M.Caron, A.Michaud, B.Lombard, S.T.da Rocha, J.Offer, D.Loew, N.Servant, M.Wassef, F.Burlina, S.J.Gamblin, E.Heard, R.Margueron.

ABSTRACT

Polycomb Group (PcG) proteins maintain transcriptional repression throughout development, mostly by regulating chromatin structure. Polycomb Repressive Complex 2 (PRC2), a component of the Polycomb machinery, is responsible for the methylation of histone H3 lysine 27 (H3K27me2/3). Jarid2 was previously identified as a cofactor of PRC2, regulating PRC2 targeting to chromatin and its enzymatic activity. Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. Here, we reveal an unexpected crosstalk between Jarid2 and PRC2, with Jarid2 being methylated by PRC2. This modification is recognized by the Eed core component of PRC2 and triggers an allosteric activation of PRC2's enzymatic activity. We show that Jarid2 methylation is important to promote PRC2 activity at a locus devoid of H3K27me3 and for the correct deposition of this mark during cell differentiation. Our results uncover a regulation loop where Jarid2 methylation fine-tunes PRC2 activity depending on the chromatin context.