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PDBsum entry 4x2m
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Transport protein
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PDB id
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4x2m
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References listed in PDB file
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Key reference
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Title
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Structural characterization of the principal mRNA-Export factor mex67-Mtr2 from chaetomium thermophilum.
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Authors
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S.Aibara,
E.Valkov,
M.H.Lamers,
L.Dimitrova,
E.Hurt,
M.Stewart.
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Ref.
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Acta Crystallogr F Struct Biol Commun, 2015,
71,
876-888.
[DOI no: ]
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PubMed id
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Abstract
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Members of the Mex67-Mtr2/NXF-NXT1 family are the principal mediators of the
nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains
(RRM, LRR, NTF2-like and UBA) that are thought to be present across species,
although the level of sequence conservation between organisms, especially in
lower eukaryotes, is low. Here, the crystal structures of these domains from the
thermophilic fungus Chaetomium thermophilum are presented together with
small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate
that, not withstanding the limited sequence conservation between different NXF
family members, the molecules retain similar structural and RNA-binding
properties. Moreover, the resolution of crystal structures obtained with the C.
thermophilum domains was often higher than that obtained previously and, when
combined with solution and biochemical studies, provided insight into the
structural organization, self-association and RNA-binding properties of
Mex67-Mtr2 that facilitate mRNA nuclear export.
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