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PDBsum entry 4x23
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Structural protein/DNA
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PDB id
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4x23
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Contents |
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95 a.a.
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79 a.a.
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102 a.a.
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90 a.a.
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22 a.a.
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21 a.a.
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References listed in PDB file
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Key reference
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Title
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A conserved mechanism for centromeric nucleosome recognition by centromere protein cenp-C.
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Authors
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H.Kato,
J.Jiang,
B.R.Zhou,
M.Rozendaal,
H.Feng,
R.Ghirlando,
T.S.Xiao,
A.F.Straight,
Y.Bai.
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Ref.
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Science, 2013,
340,
1110-1113.
[DOI no: ]
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PubMed id
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Abstract
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Chromosome segregation during mitosis requires assembly of the kinetochore
complex at the centromere. Kinetochore assembly depends on specific recognition
of the histone variant CENP-A in the centromeric nucleosome by centromere
protein C (CENP-C). We have defined the determinants of this recognition
mechanism and discovered that CENP-C binds a hydrophobic region in the CENP-A
tail and docks onto the acidic patch of histone H2A and H2B. We further found
that the more broadly conserved CENP-C motif uses the same mechanism for CENP-A
nucleosome recognition. Our findings reveal a conserved mechanism for protein
recruitment to centromeres and a histone recognition mode whereby a disordered
peptide binds the histone tail through hydrophobic interactions facilitated by
nucleosome docking.
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