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PDBsum entry 4wxj
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Membrane protein
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PDB id
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4wxj
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References listed in PDB file
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Key reference
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Title
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Functional reconstitution of drosophila melanogaster nmj glutamate receptors.
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Authors
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T.H.Han,
P.Dharkar,
M.L.Mayer,
M.Serpe.
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Ref.
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Proc Natl Acad Sci U S A, 2015,
112,
6182-6187.
[DOI no: ]
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PubMed id
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Abstract
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The Drosophila larval neuromuscular junction (NMJ), at which glutamate acts as
the excitatory neurotransmitter, is a widely used model for genetic analysis of
synapse function and development. Despite decades of study, the inability to
reconstitute NMJ glutamate receptor function using heterologous expression
systems has complicated the analysis of receptor function, such that it is
difficult to resolve the molecular basis for compound phenotypes observed in
mutant flies. We find that Drosophila Neto functions as an essential component
required for the function of NMJ glutamate receptors, permitting analysis of
glutamate receptor responses in Xenopus oocytes. In combination with a
crystallographic analysis of the GluRIIB ligand binding domain, we use this
system to characterize the subunit dependence of assembly, channel block, and
ligand selectivity for Drosophila NMJ glutamate receptors.
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