PDBsum entry 4wt8

Ribosome

PDB id: 4wt8

Contents

Protein chains

234 a.a.

206 a.a.

208 a.a.

150 a.a.

101 a.a.

155 a.a.

138 a.a.

127 a.a.

98 a.a.

119 a.a.

124 a.a.

124 a.a.

60 a.a.

88 a.a.

83 a.a.

99 a.a.

70 a.a.

78 a.a.

99 a.a.

24 a.a.

DNA/RNA

Ligands

PAR

References listed in PDB file

Key reference

• Title: Bactobolin a binds to a site on the 70s ribosome distinct from previously seen antibiotics.
• Authors: A.Amunts, K.Fiedorczuk, T.T.Truong, J.Chandler, E.Peter greenberg, V.Ramakrishnan.
• Ref.: J Mol Biol, 2015, 427, 753-755. [DOI no: 10.1016/j.jmb.2014.12.018]
• PubMed id: 25562208

Abstract

The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.