PDBsum entry 4wpx

Cell cycle

PDB id: 4wpx

Contents

Protein chains

171 a.a.

81 a.a.

108 a.a.

117 a.a.

PDB id:

4wpx

Name:

Cell cycle

Title:

Chaetomium theromophilum trex2 cid domain complex

Structure:

Cell division control protein 31-like protein. Chain: a, d. Synonym: cdc31. Engineered: yes. Putative sac3 family protein. Chain: b, e. Fragment: unp residues 1085-1170. Engineered: yes. Putative uncharacterized protein.

Source:

Chaetomium thermophilum. Organism_taxid: 209285. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

3.31Å R-factor: 0.221 R-free: 0.277

Authors:

E.Valkov,M.Stewart

Key ref:

L.Dimitrova et al. (2015). Structural Characterization of the Chaetomium thermophilum TREX-2 Complex and its Interaction with the mRNA Nuclear Export Factor Mex67:Mtr2. Structure, 23, 1246-1257. PubMed id: 26051714 DOI: 10.1016/j.str.2015.05.002

Date:

21-Oct-14 Release date: 17-Jun-15

Protein chains

G0SAR7  (G0SAR7_CHATD) -  Cell division control protein 31-like protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 177 a.a.

Struc: 171 a.a.

Protein chains

G0SGL4  (G0SGL4_CHATD) -  Putative SAC3 family protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 1716 a.a.

Struc: 81 a.a.

Protein chain

G0S6Y1  (G0S6Y1_CHATD) -  Uncharacterized protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 168 a.a.

Struc: 108 a.a.

Protein chain

G0S6Y1  (G0S6Y1_CHATD) -  Uncharacterized protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 168 a.a.

Struc: 117 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.?

DOI no: 10.1016/j.str.2015.05.002

Structure 23:1246-1257 (2015)

PubMed id: 26051714

Structural Characterization of the Chaetomium thermophilum TREX-2 Complex and its Interaction with the mRNA Nuclear Export Factor Mex67:Mtr2.

L.Dimitrova, E.Valkov, S.Aibara, D.Flemming, S.H.McLaughlin, E.Hurt, M.Stewart.

ABSTRACT

The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67(NTF2L)) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance.