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PDBsum entry 4wju
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Biosynthetic protein
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PDB id
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4wju
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References listed in PDB file
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Key reference
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Title
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A network of assembly factors is involved in remodeling rrna elements during preribosome maturation.
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Authors
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J.Baßler,
H.Paternoga,
I.Holdermann,
M.Thoms,
S.Granneman,
C.Barrio-Garcia,
A.Nyarko,
W.Lee,
G.Stier,
S.A.Clark,
D.Schraivogel,
M.Kallas,
R.Beckmann,
D.Tollervey,
E.Barbar,
I.Sinning,
E.Hurt.
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Ref.
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J Cell Biol, 2014,
207,
481-498.
[DOI no: ]
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PubMed id
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Abstract
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Eukaryotic ribosome biogenesis involves ∼200 assembly factors, but how these
contribute to ribosome maturation is poorly understood. Here, we identify a
network of factors on the nascent 60S subunit that actively remodels preribosome
structure. At its hub is Rsa4, a direct substrate of the force-generating ATPase
Rea1. We show that Rsa4 is connected to the central protuberance by binding to
Rpl5 and to ribosomal RNA (rRNA) helix 89 of the nascent peptidyl transferase
center (PTC) through Nsa2. Importantly, Nsa2 binds to helix 89 before relocation
of helix 89 to the PTC. Structure-based mutations of these factors reveal the
functional importance of their interactions for ribosome assembly. Thus, Rsa4 is
held tightly in the preribosome and can serve as a "distribution box,"
transmitting remodeling energy from Rea1 into the developing ribosome. We
suggest that a relay-like factor network coupled to a mechano-enzyme is
strategically positioned to relocate rRNA elements during ribosome maturation.
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