PDBsum entry 4wa6

Hydrolase/transcription

PDB id: 4wa6

Contents

Protein chains

440 a.a.

96 a.a.

82 a.a.

85 a.a.

415 a.a.

71 a.a.

82 a.a.

Metals

_ZN ×15

Waters ×139

PDB id:

4wa6

Name:

Hydrolase/transcription

Title:

Structure of yeast saga dubm with sgf73 n59d mutant at 2.36 angstroms resolution

Structure:

Ubiquitin carboxyl-terminal hydrolase 8. Chain: a, d. Synonym: deubiquitinating enzyme 8,ubiquitin thioesterase 8, ubiquitin-specific-processing protease 8. Engineered: yes. Transcription and mRNA export factor sus1. Chain: b, f. Engineered: yes. Saga-associated factor 11.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: ubp8, ymr223w, ym9959.05. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sus1, ybr111w-a. Organism_taxid: 307796.

Resolution:

2.36Å R-factor: 0.186 R-free: 0.244

Authors:

C.Wolberger,M.Yan

Key ref:

C.Wolberger and m.yan Structure of yeast saga dubm with sgf73 n59d mutant a angstroms resolution. To be published, .

Date:

28-Aug-14 Release date: 04-Mar-15

Protein chain

P50102  (UBP8_YEAST) -  Ubiquitin carboxyl-terminal hydrolase 8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 471 a.a.

Struc: 440 a.a.

Protein chains

Q6WNK7  (SUS1_YEAST) -  Transcription and mRNA export factor SUS1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 96 a.a.

Struc: 96 a.a.

Protein chain

A6ZWK1  (SGF11_YEAS7) -  SAGA-associated factor 11 from Saccharomyces cerevisiae (strain YJM789)

Seq: 99 a.a.

Struc: 82 a.a.

Protein chain

P53165  (SGF73_YEAST) -  SAGA-associated factor 73 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 657 a.a.

Struc: 85 a.a.*

Protein chain

P50102  (UBP8_YEAST) -  Ubiquitin carboxyl-terminal hydrolase 8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 471 a.a.

Struc: 415 a.a.

Protein chain

A6ZWK1  (SGF11_YEAS7) -  SAGA-associated factor 11 from Saccharomyces cerevisiae (strain YJM789)

Seq: 99 a.a.

Struc: 71 a.a.

Protein chain

P53165  (SGF73_YEAST) -  SAGA-associated factor 73 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 657 a.a.

Struc: 82 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, D: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
• Enzyme class 3: Chains B, C, E, F, G, H: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.