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PDBsum entry 4v1o
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Transcription
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PDB id
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4v1o
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Contents |
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1422 a.a.
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1156 a.a.
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266 a.a.
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178 a.a.
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214 a.a.
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84 a.a.
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171 a.a.
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133 a.a.
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119 a.a.
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65 a.a.
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115 a.a.
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46 a.a.
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300 a.a.
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180 a.a.
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122 a.a.
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105 a.a.
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139 a.a.
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158 a.a.
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99 a.a.
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419 a.a.
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232 a.a.
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203 a.a.
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88 a.a.
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References listed in PDB file
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Key reference
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Title
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Architecture of the RNA polymerase ii-Mediator core initiation complex.
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Authors
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C.Plaschka,
L.Larivière,
L.Wenzeck,
M.Seizl,
M.Hemann,
D.Tegunov,
E.V.Petrotchenko,
C.H.Borchers,
W.Baumeister,
F.Herzog,
E.Villa,
P.Cramer.
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Ref.
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Nature, 2015,
518,
376-380.
[DOI no: ]
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PubMed id
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Abstract
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The conserved co-activator complex Mediator enables regulated transcription
initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit
core Mediator (cMed) comprising all essential Mediator subunits from
Saccharomyces cerevisiae. The cryo-electron microscopic structure of cMed bound
to a core initiation complex was determined at 9.7 Å resolution. cMed binds
Pol II around the Rpb4-Rpb7 stalk near the carboxy-terminal domain (CTD). The
Mediator head module binds the Pol II dock and the TFIIB ribbon and stabilizes
the initiation complex. The Mediator middle module extends to the Pol II foot
with a 'plank' that may influence polymerase conformation. The Mediator subunit
Med14 forms a 'beam' between the head and middle modules and connects to the
tail module that is predicted to bind transcription activators located on
upstream DNA. The Mediator 'arm' and 'hook' domains contribute to a 'cradle'
that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.
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