PDBsum entry 4uww

Structural protein

PDB id: 4uww

Contents

Protein chain

130 a.a.

Waters ×74

PDB id:

4uww

Name:

Structural protein

Title:

Crystallographic structure of the intramineral protein struthicalcin from struthio camelus eggshell

Structure:

Struthiocalcin-1. Chain: a. Synonym: sca-1

Source:

Struthio camelus. African ostrich. Organism_taxid: 8801

Resolution:

1.44Å R-factor: 0.214 R-free: 0.232

Authors:

R.R.Ruiz,A.Moreno,A.Romero

Key ref:

R.R.Ruiz-Arellano et al. (2015). Structure of struthiocalcin-1, an intramineral protein from Struthio camelus eggshell, in two crystal forms. Acta Crystallogr D Biol Crystallogr, 71, 809-818. PubMed id: 25849392 DOI: 10.1107/S139900471500125X

Date:

14-Aug-14 Release date: 08-Apr-15

Protein chain

P83514  (SCAL1_STRCA) -  Struthiocalcin-1 from Struthio camelus

Seq: 132 a.a.

Struc: 130 a.a.

DOI no: 10.1107/S139900471500125X

Acta Crystallogr D Biol Crystallogr 71:809-818 (2015)

PubMed id: 25849392

Structure of struthiocalcin-1, an intramineral protein from Struthio camelus eggshell, in two crystal forms.

R.R.Ruiz-Arellano, F.J.Medrano, A.Moreno, A.Romero.

ABSTRACT

Biomineralization is the process by which living organisms produce minerals. One remarkable example is the formation of eggshells in birds. Struthiocalcins present in the ostrich (Struthio camellus) eggshell matrix act as biosensors of calcite growth during eggshell formation. Here, the crystal structure of struthiocalcin-1 (SCA-1) is reported in two different crystal forms. The structure is a compact single domain with an α/β fold characteristic of the C-type lectin family. In contrast to the related avian ovocleidin OC17, the electrostatic potential on the molecular surface is dominated by an acidic patch. Scanning electron microscopy combined with Raman spectroscopy indicates that these intramineral proteins (SCA-1 and SCA-2) induce calcium carbonate precipitation, leading to the formation of a stable form of calcite in the mature eggshell. Finally, the implications of these two intramineral proteins SCA-1 and SCA-2 in the nucleation of calcite during the formation of eggshells in ratite birds are discussed.