PDBsum entry 4uvk

Cell cycle

PDB id: 4uvk

Contents

Protein chain

876 a.a.

Waters ×209

PDB id:

4uvk

Name:

Cell cycle

Title:

Cohesin subunit scc3 from z. Rouxii, 88-1035

Structure:

Zyro0d15994p. Chain: a. Fragment: scc3 his-tagged, unp residues 88-1035. Synonym: scc3. Engineered: yes

Source:

Zygosaccharomyces rouxii. Organism_taxid: 4956. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: c41

Resolution:

2.60Å R-factor: 0.190 R-free: 0.245

Authors:

M.B.Roig,K.Nasmyth,J.Lowe

Key ref:

M.B.Roig et al. (2014). Structure and function of cohesin's Scc3/SA regulatory subunit. Febs Lett, 588, 3692-3702. PubMed id: 25171859 DOI: 10.1016/j.febslet.2014.08.015

Date:

06-Aug-14 Release date: 20-Aug-14

Protein chain

C5DWM3  (C5DWM3_ZYGRC) -  ZYRO0D15994p from Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229)

Seq: 1080 a.a.

Struc: 876 a.a.

DOI no: 10.1016/j.febslet.2014.08.015

Febs Lett 588:3692-3702 (2014)

PubMed id: 25171859

Structure and function of cohesin's Scc3/SA regulatory subunit.

M.B.Roig, J.Löwe, K.L.Chan, F.Beckouët, J.Metson, K.Nasmyth.

ABSTRACT

Sister chromatid cohesion involves entrapment of sister DNAs by a cohesin ring created through association of a kleisin subunit (Scc1) with ATPase heads of Smc1/Smc3 heterodimers. Cohesin's association with chromatin involves subunits recruited by Scc1: Wapl, Pds5, and Scc3/SA, in addition to Scc2/4 loading complex. Unlike Pds5, Wapl, and Scc2/4, Scc3s are encoded by all eukaryotic genomes. Here, a crystal structure of Scc3 reveals a hook-shaped protein composed of tandem α helices. Its N-terminal domain contains a conserved and essential surface (CES) present even in organisms lacking Pds5, Wapl, and Scc2/4, while its C-terminal domain binds a section of the kleisin Scc1. Scc3 turns over in G2/M while maintaining cohesin's association with chromosomes and it promotes de-acetylation of Smc3 upon Scc1 cleavage.