PDBsum entry 4uvj

Cell cycle

PDB id: 4uvj

Contents

Protein chains

364 a.a.

Waters ×401

PDB id:

4uvj

Name:

Cell cycle

Title:

Cohesin subunit scc3 from yeast, 674-1072

Structure:

Cohesin subunit scc3. Chain: a, b. Fragment: c-terminal domain, unp residues 674-1072. Synonym: irregular cell behavior protein 1, scc3. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: c41

Resolution:

2.10Å R-factor: 0.189 R-free: 0.247

Authors:

M.B.Roig,K.Nasmyth,J.Lowe

Key ref:

M.B.Roig et al. (2014). Structure and function of cohesin's Scc3/SA regulatory subunit. Febs Lett, 588, 3692-3702. PubMed id: 25171859 DOI: 10.1016/j.febslet.2014.08.015

Date:

06-Aug-14 Release date: 20-Aug-14

Protein chains

P40541  (SCC3_YEAST) -  Cohesin subunit SCC3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1150 a.a.

Struc: 364 a.a.

DOI no: 10.1016/j.febslet.2014.08.015

Febs Lett 588:3692-3702 (2014)

PubMed id: 25171859

Structure and function of cohesin's Scc3/SA regulatory subunit.

M.B.Roig, J.Löwe, K.L.Chan, F.Beckouët, J.Metson, K.Nasmyth.

ABSTRACT

Sister chromatid cohesion involves entrapment of sister DNAs by a cohesin ring created through association of a kleisin subunit (Scc1) with ATPase heads of Smc1/Smc3 heterodimers. Cohesin's association with chromatin involves subunits recruited by Scc1: Wapl, Pds5, and Scc3/SA, in addition to Scc2/4 loading complex. Unlike Pds5, Wapl, and Scc2/4, Scc3s are encoded by all eukaryotic genomes. Here, a crystal structure of Scc3 reveals a hook-shaped protein composed of tandem α helices. Its N-terminal domain contains a conserved and essential surface (CES) present even in organisms lacking Pds5, Wapl, and Scc2/4, while its C-terminal domain binds a section of the kleisin Scc1. Scc3 turns over in G2/M while maintaining cohesin's association with chromosomes and it promotes de-acetylation of Smc3 upon Scc1 cleavage.