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PDBsum entry 4ui9
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protein metals Protein-protein interface(s) links
Cell cycle PDB id
4ui9

 

 

 

 

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Contents
Protein chains
1441 a.a.
84 a.a.
524 a.a.
55 a.a.
56 a.a.
498 a.a.
25 a.a.
730 a.a.
504 a.a.
182 a.a.
59 a.a.
631 a.a.
685 a.a.
491 a.a.
387 a.a.
94 a.a.
21 a.a.
24 a.a.
25 a.a.
484 a.a.
Metals
_ZN ×5
PDB id:
4ui9
Name: Cell cycle
Title: Atomic structure of the human anaphase-promoting complex
Structure: Anaphase-promoting complex subunit 1. Chain: a. Synonym: apc1, cyclosome subunit 1, mitotic checkpoint regulator, testis-specific gene 24 protein, apc1, cyclosome subunit 1, mitotic checkpoint regulator, testis-specific gene 24 protein. Anaphase-promoting complex subunit 11. Chain: b. Synonym: apc11, cyclosome subunit 11, hepatocellular carcinoma- associated ring finger protein, apc11, cyclosome subunit 11,
Source: Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606
Authors: L.Chang,Z.Zhang,J.Yang,S.H.Mclaughlin,D.Barford
Key ref: L.Chang et al. (2015). Atomic structure of the APC/C and its mechanism of protein ubiquitination. Nature, 522, 450-454. PubMed id: 26083744 DOI: 10.1038/nature14471
Date:
27-Mar-15     Release date:   17-Jun-15    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9H1A4  (APC1_HUMAN) -  Anaphase-promoting complex subunit 1 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1944 a.a.
1441 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9NYG5  (APC11_HUMAN) -  Anaphase-promoting complex subunit 11 from Homo sapiens
Seq:
Struc: 		84 a.a.
84 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9UJX2  (CDC23_HUMAN) -  Cell division cycle protein 23 homolog from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		597 a.a.
524 a.a.*
Protein chain
Pfam   ArchSchema ?
P60006  (APC15_HUMAN) -  Anaphase-promoting complex subunit 15 from Homo sapiens
Seq:
Struc: 		121 a.a.
55 a.a.*
Protein chain
Pfam   ArchSchema ?
Q96DE5  (APC16_HUMAN) -  Anaphase-promoting complex subunit 16 from Homo sapiens
Seq:
Struc: 		110 a.a.
56 a.a.
Protein chains
Pfam   ArchSchema ?
P30260  (CDC27_HUMAN) -  Cell division cycle protein 27 homolog from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		824 a.a.
498 a.a.*
Protein chain
Pfam   ArchSchema ?
Q8NHZ8  (CDC26_HUMAN) -  Anaphase-promoting complex subunit CDC26 from Homo sapiens
Seq:
Struc: 		85 a.a.
25 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9UJX5  (APC4_HUMAN) -  Anaphase-promoting complex subunit 4 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		808 a.a.
730 a.a.*
Protein chains
Pfam   ArchSchema ?
Q13042  (CDC16_HUMAN) -  Cell division cycle protein 16 homolog from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		620 a.a.
504 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9UM13  (APC10_HUMAN) -  Anaphase-promoting complex subunit 10 from Homo sapiens
Seq:
Struc: 		185 a.a.
182 a.a.
Protein chain
Pfam   ArchSchema ?
Q9BS18  (APC13_HUMAN) -  Anaphase-promoting complex subunit 13 from Homo sapiens
Seq:
Struc: 		74 a.a.
59 a.a.
Protein chain
Pfam   ArchSchema ?
Q9UJX6  (ANC2_HUMAN) -  Anaphase-promoting complex subunit 2 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		822 a.a.
631 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9UJX4  (APC5_HUMAN) -  Anaphase-promoting complex subunit 5 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		755 a.a.
685 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9UJX2  (CDC23_HUMAN) -  Cell division cycle protein 23 homolog from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		597 a.a.
491 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9UM11  (FZR1_HUMAN) -  Fizzy-related protein homolog from Homo sapiens
Seq:
Struc: 		496 a.a.
387 a.a.
Protein chain
Pfam   ArchSchema ?
Q9UKT4  (FBX5_HUMAN) -  F-box only protein 5 from Homo sapiens
Seq:
Struc: 		447 a.a.
94 a.a.*
Protein chain
No UniProt id for this chain
Struc: 21 a.a.
Protein chain
Pfam   ArchSchema ?
Q9UKT4  (FBX5_HUMAN) -  F-box only protein 5 from Homo sapiens
Seq:
Struc: 		447 a.a.
24 a.a.*
Protein chain
Pfam   ArchSchema ?
Q8NHZ8  (CDC26_HUMAN) -  Anaphase-promoting complex subunit CDC26 from Homo sapiens
Seq:
Struc: 		85 a.a.
25 a.a.
Protein chains
Pfam   ArchSchema ?
Q9UJX3  (APC7_HUMAN) -  Anaphase-promoting complex subunit 7 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		565 a.a.
484 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 42 residue positions (black crosses)

 

 
DOI no: 10.1038/nature14471 Nature 522:450-454 (2015)
PubMed id: 26083744  
 
 
Atomic structure of the APC/C and its mechanism of protein ubiquitination.
L.Chang, Z.Zhang, J.Yang, S.H.McLaughlin, D.Barford.
 
  ABSTRACT  
 
The anaphase-promoting complex (APC/C) is a multimeric RING E3 ubiquitin ligase that controls chromosome segregation and mitotic exit. Its regulation by coactivator subunits, phosphorylation, the mitotic checkpoint complex and interphase early mitotic inhibitor 1 (Emi1) ensures the correct order and timing of distinct cell-cycle transitions. Here we use cryo-electron microscopy to determine atomic structures of APC/C-coactivator complexes with either Emi1 or a UbcH10-ubiquitin conjugate. These structures define the architecture of all APC/C subunits, the position of the catalytic module and explain how Emi1 mediates inhibition of the two E2s UbcH10 and Ube2S. Definition of Cdh1 interactions with the APC/C indicates how they are antagonized by Cdh1 phosphorylation. The structure of the APC/C with UbcH10-ubiquitin reveals insights into the initiating ubiquitination reaction. Our results provide a quantitative framework for the design of future experiments to investigate APC/C functions in vivo.
 

 

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