PDBsum entry 4uer

Translation

PDB id: 4uer

Contents

Protein chains

100 a.a.

63 a.a.

188 a.a.

184 a.a.

214 a.a.

97 a.a.

81 a.a.

96 a.a.

70 a.a.

71 a.a.

206 a.a.

223 a.a.

185 a.a.

217 a.a.

83 a.a.

206 a.a.

129 a.a.

141 a.a.

107 a.a.

127 a.a.

144 a.a.

145 a.a.

53 a.a.

150 a.a.

134 a.a.

155 a.a.

318 a.a.

124 a.a.

143 a.a.

124 a.a.

120 a.a.

260 a.a.

60 a.a.

226 a.a.

87 a.a.

449 a.a.

572 a.a.

544 a.a.

DNA/RNA

Metals

_ZN ×4

References listed in PDB file

Key reference

• Title: Structure of a yeast 40s-Eif1-Eif1a-Eif3-Eif3j initiation complex.
• Authors: C.H.Aylett, D.Boehringer, J.P.Erzberger, T.Schaefer, N.Ban.
• Ref.: Nat Struct Biol, 2015, 22, 269-271. [DOI no: 10.1038/nsmb.2963]
• PubMed id: 25664723

Abstract

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.