PDBsum entry 4uer

Translation

PDB id: 4uer

Contents

Protein chains

100 a.a.

63 a.a.

188 a.a.

184 a.a.

214 a.a.

97 a.a.

81 a.a.

96 a.a.

70 a.a.

71 a.a.

206 a.a.

223 a.a.

185 a.a.

217 a.a.

83 a.a.

206 a.a.

129 a.a.

141 a.a.

107 a.a.

127 a.a.

144 a.a.

145 a.a.

53 a.a.

150 a.a.

134 a.a.

155 a.a.

318 a.a.

124 a.a.

143 a.a.

124 a.a.

120 a.a.

260 a.a.

60 a.a.

226 a.a.

87 a.a.

449 a.a.

572 a.a.

544 a.a.

DNA/RNA

Metals

_ZN ×4

PDB id:

4uer

Name:

Translation

Title:

40s-eif1-eif1a-eif3-eif3j translation initiation complex from lachancea kluyveri

Structure:

Eif1a. Chain: 0. Es28. Chain: 1. Es8. Chain: 2. Es7. Chain: 3. Es1.

Source:

Lachancea kluyveri. Organism_taxid: 4934. Organism_taxid: 4934

Authors:

C.H.S.Aylett,D.Boehringer,J.P.Erzberger,T.Schaefer,N.Ban

Key ref:

C.H.Aylett et al. (2015). Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex. Nat Struct Biol, 22, 269-271. PubMed id: 25664723 DOI: 10.1038/nsmb.2963

Date:

18-Dec-14 Release date: 11-Feb-15

Protein chain

A0A0J9X202  (A0A0J9X202_LACKL) -  Eukaryotic translation initiation factor 4C from Lachancea kluyveri

Seq: 100 a.a.

Struc: 100 a.a.

Protein chain

A0A0J9X212  (A0A0J9X212_LACKL) -  ES28 from Lachancea kluyveri

Seq: 63 a.a.

Struc: 63 a.a.

Protein chain

A0A0J9X221  (A0A0J9X221_LACKL) -  40S ribosomal protein S8 from Lachancea kluyveri

Seq: 188 a.a.

Struc: 188 a.a.

Protein chain

A0A0J9X232  (A0A0J9X232_LACKL) -  40S ribosomal protein S7 from Lachancea kluyveri

Seq: 184 a.a.

Struc: 184 a.a.

Protein chain

A0A0J9X233  (A0A0J9X233_LACKL) -  Small ribosomal subunit protein eS1 from Lachancea kluyveri

Seq: 214 a.a.

Struc: 214 a.a.

Protein chain

A0A0J9X234  (A0A0J9X234_LACKL) -  40S ribosomal protein S26 from Lachancea kluyveri

Seq: 97 a.a.

Struc: 97 a.a.

Protein chain

A0A0J9X235  (A0A0J9X235_LACKL) -  40S ribosomal protein S27 from Lachancea kluyveri

Seq: 81 a.a.

Struc: 81 a.a.

Protein chain

A0A0J9X236  (A0A0J9X236_LACKL) -  ES10 from Lachancea kluyveri

Seq: 96 a.a.

Struc: 96 a.a.

Protein chain

A0A0J9X237  (A0A0J9X237_LACKL) -  40S ribosomal protein S25 from Lachancea kluyveri

Seq: 70 a.a.

Struc: 70 a.a.

Protein chain

A0A0J9X203  (A0A0J9X203_LACKL) -  ES31 from Lachancea kluyveri

Seq: 71 a.a.

Struc: 71 a.a.

Protein chain

A0A0J9X204  (A0A0J9X204_LACKL) -  Small ribosomal subunit protein uS2 from Lachancea kluyveri

Seq: 206 a.a.

Struc: 206 a.a.

Protein chain

A0A0J9X205  (A0A0J9X205_LACKL) -  40S ribosomal protein S3 from Lachancea kluyveri

Seq: 223 a.a.

Struc: 223 a.a.

Protein chain

A0A0J9X206  (A0A0J9X206_LACKL) -  US4 from Lachancea kluyveri

Seq: 185 a.a.

Struc: 185 a.a.

Protein chain

A0A0J9X207  (A0A0J9X207_LACKL) -  Small ribosomal subunit protein uS5 from Lachancea kluyveri

Seq: 217 a.a.

Struc: 217 a.a.

Protein chain

A0A0J9X208  (A0A0J9X208_LACKL) -  eIF1 from Lachancea kluyveri

Seq: 83 a.a.

Struc: 83 a.a.

Protein chain

A0A0J9X209  (A0A0J9X209_LACKL) -  US7 from Lachancea kluyveri

Seq: 206 a.a.

Struc: 206 a.a.

Protein chain

A0A0J9X210  (A0A0J9X210_LACKL) -  US8 from Lachancea kluyveri

Seq: 129 a.a.

Struc: 129 a.a.

Protein chain

A0A0J9X211  (A0A0J9X211_LACKL) -  US9 from Lachancea kluyveri

Seq: 141 a.a.

Struc: 141 a.a.

Protein chain

A0A0J9X213  (A0A0J9X213_LACKL) -  US10 from Lachancea kluyveri

Seq: 107 a.a.

Struc: 107 a.a.

Protein chain

A0A0J9X214  (A0A0J9X214_LACKL) -  US11 from Lachancea kluyveri

Seq: 127 a.a.

Struc: 127 a.a.

Protein chain

Q875Q2  (Q875Q2_LACKL) -  RPS23 from Lachancea kluyveri

Seq: 145 a.a.

Struc: 144 a.a.*

Protein chain

A0A0J9X215  (A0A0J9X215_LACKL) -  US13 from Lachancea kluyveri

Seq: 145 a.a.

Struc: 145 a.a.

Protein chain

A0A0J9X216  (A0A0J9X216_LACKL) -  US14 from Lachancea kluyveri

Seq: 53 a.a.

Struc: 53 a.a.

Protein chain

A0A0J9X217  (A0A0J9X217_LACKL) -  US15 from Lachancea kluyveri

Seq: 150 a.a.

Struc: 150 a.a.

Protein chain

Q875Q1  (Q875Q1_LACKL) -  40S ribosomal protein S24 from Lachancea kluyveri

Seq: 135 a.a.

Struc: 134 a.a.*

Protein chain

A0A0J9X218  (A0A0J9X218_LACKL) -  US17 from Lachancea kluyveri

Seq: 155 a.a.

Struc: 155 a.a.

Protein chain

A0A0J9X219  (A0A0J9X219_LACKL) -  Rack1 from Lachancea kluyveri

Seq: 318 a.a.

Struc: 318 a.a.

Protein chain

A0A0J9X220  (A0A0J9X220_LACKL) -  US19 from Lachancea kluyveri

Seq: 124 a.a.

Struc: 124 a.a.

Protein chain

A0A0J9X222  (A0A0J9X222_LACKL) -  ES19 from Lachancea kluyveri

Seq: 143 a.a.

Struc: 143 a.a.

Protein chain

A0A0J9X223  (A0A0J9X223_LACKL) -  40S ribosomal protein S12 from Lachancea kluyveri

Seq: 124 a.a.

Struc: 124 a.a.

Protein chain

A0A0J9X224  (A0A0J9X224_LACKL) -  ES17 from Lachancea kluyveri

Seq: 120 a.a.

Struc: 120 a.a.

Protein chain

A0A0J9X225  (A0A0J9X225_LACKL) -  40S ribosomal protein S4 from Lachancea kluyveri

Seq: 260 a.a.

Struc: 260 a.a.

Protein chain

A0A0J9X226  (A0A0J9X226_LACKL) -  40S ribosomal protein S30 from Lachancea kluyveri

Seq: 60 a.a.

Struc: 60 a.a.

Protein chain

A0A0J9X227  (A0A0J9X227_LACKL) -  40S ribosomal protein S6 from Lachancea kluyveri

Seq: 226 a.a.

Struc: 226 a.a.

Protein chain

A0A0J9X228  (A0A0J9X228_LACKL) -  40S ribosomal protein S21 from Lachancea kluyveri

Seq: 87 a.a.

Struc: 87 a.a.

Protein chain

A0A0J9X229  (A0A0J9X229_LACKL) -  Eukaryotic translation initiation factor 3 subunit A from Lachancea kluyveri

Seq: 964 a.a.

Struc: 449 a.a.

Protein chain

A0A0J9X230  (A0A0J9X230_LACKL) -  Eukaryotic translation initiation factor 3 subunit B from Lachancea kluyveri

Seq: 763 a.a.

Struc: 572 a.a.*

Protein chain

A0A0J9X231  (A0A0J9X231_LACKL) -  Eukaryotic translation initiation factor 3 subunit C from Lachancea kluyveri

Seq: 812 a.a.

Struc: 544 a.a.

* PDB and UniProt seqs differ at 20 residue positions (black crosses)

DNA/RNA chain

U-A-U-C-U-G-G-U-U-G-A-U-C-C-U-G-C-C-A-G-U-A-G-U-C-A-U-A-U-G-C-U-U-G-U-C-U-C-A- ... 1781 bases

DOI no: 10.1038/nsmb.2963

Nat Struct Biol 22:269-271 (2015)

PubMed id: 25664723

Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex.

C.H.Aylett, D.Boehringer, J.P.Erzberger, T.Schaefer, N.Ban.

ABSTRACT

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.