PDBsum entry 4u3j

Structural protein/protein binding

PDB id: 4u3j

Contents

Protein chains

431 a.a.

417 a.a.

241 a.a.

Ligands

GTP ×2

Metals

_MG ×2

Waters ×21

PDB id:

4u3j

Name:

Structural protein/protein binding

Title:

Tog2:alpha/beta-tubulin complex

Structure:

Tubulin alpha-1 chain. Chain: a. Engineered: yes. Tubulin beta chain. Chain: b. Synonym: beta-tubulin. Engineered: yes. Mutation: yes. Protein stu2.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: tub1. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932. Gene: tub2. Gene: stu2. Expressed in: escherichia coli.

Resolution:

2.81Å R-factor: 0.220 R-free: 0.259

Authors:

P.Ayaz,L.M.Rice

Key ref:

P.Ayaz et al. (2014). A tethered delivery mechanism explains the catalytic action of a microtubule polymerase. Elife, 3, e03069. PubMed id: 25097237 DOI: 10.7554/eLife.03069

Date:

22-Jul-14 Release date: 20-Aug-14

Protein chain

P09733  (TBA1_YEAST) -  Tubulin alpha-1 chain from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 447 a.a.

Struc: 431 a.a.

Protein chain

P02557  (TBB_YEAST) -  Tubulin beta chain from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 457 a.a.

Struc: 417 a.a.*

Protein chain

P46675  (STU2_YEAST) -  Protein STU2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 888 a.a.

Struc: 241 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????

DOI no: 10.7554/eLife.03069

Elife 3:e03069 (2014)

PubMed id: 25097237

A tethered delivery mechanism explains the catalytic action of a microtubule polymerase.

P.Ayaz, S.Munyoki, E.A.Geyer, F.A.Piedra, E.S.Vu, R.Bromberg, Z.Otwinowski, N.V.Grishin, C.A.Brautigam, L.M.Rice.

ABSTRACT

No abstract given.