PDBsum entry 4u2m

Transcription

PDB id: 4u2m

Contents

Protein chains

228 a.a.

240 a.a.

Waters ×163

PDB id:

4u2m

Name:

Transcription

Title:

Crystal structure of a complex of the miz1- and bcl6 poz domains.

Structure:

Zinc finger and btb domain-containing protein 17,b-cell lymphoma 6 protein. Chain: a, b, c, d. Fragment: poz domain, unp residues 2-115,poz domain, unp residues 5- 129. Synonym: myc-interacting zinc finger protein 1,miz-1,zinc finger protein 151,zinc finger protein 60,bcl-6,b-cell lymphoma 5 protein, bcl-5,protein laz-3,zinc finger and btb domain-containing protein 27, zinc finger protein 51.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: zbtb17, miz1, znf151, znf60, bcl6, bcl5, laz3, zbtb27, znf51. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.23Å R-factor: 0.213 R-free: 0.240

Authors:

M.A.Stead,S.C.Wright

Key ref:

M.A.Stead and S.C.Wright (2014). Structures of heterodimeric POZ domains of Miz1/BCL6 and Miz1/NAC1. Acta Crystallogr F Struct Biol Commun, 70, 1591-1596. PubMed id: 25484205 DOI: 10.1107/S2053230X14023449

Date:

17-Jul-14 Release date: 10-Dec-14

Protein chain

P41182  (BCL6_HUMAN) -  B-cell lymphoma 6 protein from Homo sapiens

Seq: 706 a.a.

Struc: 228 a.a.*

Protein chain

Q13105  (ZBT17_HUMAN) -  Zinc finger and BTB domain-containing protein 17 from Homo sapiens

Seq: 803 a.a.

Struc: 228 a.a.*

Protein chains

P41182  (BCL6_HUMAN) -  B-cell lymphoma 6 protein from Homo sapiens

Seq: 706 a.a.

Struc: 240 a.a.*

Protein chains

Q13105  (ZBT17_HUMAN) -  Zinc finger and BTB domain-containing protein 17 from Homo sapiens

Seq: 803 a.a.

Struc: 240 a.a.*

* PDB and UniProt seqs differ at 249 residue positions (black crosses)

DOI no: 10.1107/S2053230X14023449

Acta Crystallogr F Struct Biol Commun 70:1591-1596 (2014)

PubMed id: 25484205

Structures of heterodimeric POZ domains of Miz1/BCL6 and Miz1/NAC1.

M.A.Stead, S.C.Wright.

ABSTRACT

The POZ domain is an evolutionarily conserved protein-protein interaction domain that is found in approximately 40 mammalian transcription factors. POZ domains mediate both homodimerization and the heteromeric interactions of different POZ-domain transcription factors with each other. Miz1 is a POZ-domain transcription factor that regulates cell-cycle arrest and DNA-damage responses. The activities of Miz1 are altered by its interaction with the POZ-domain transcriptional repressors BCL6 and NAC1, and these interactions have been implicated in tumourigenesis in B-cell lymphomas and in ovarian serous carcinomas that overexpress BCL6 and NAC1, respectively. A strategy for the purification of tethered POZ domains that form forced heterodimers is described, and crystal structures of the heterodimeric POZ domains of Miz1/BCL6 and of Miz1/NAC1 are reported. These structures will be relevant for the design of therapeutics that target POZ-domain interaction interfaces.