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PDBsum entry 4tzo
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Peptide binding protein
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PDB id
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4tzo
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References listed in PDB file
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Key reference
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Title
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The chromosome axis controls meiotic events through a hierarchical assembly of horma domain proteins.
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Authors
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Y.Kim,
S.C.Rosenberg,
C.L.Kugel,
N.Kostow,
O.Rog,
V.Davydov,
T.Y.Su,
A.F.Dernburg,
K.D.Corbett.
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Ref.
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Dev Cell, 2014,
31,
487-502.
[DOI no: ]
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PubMed id
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Abstract
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Proteins of the HORMA domain family play central, but poorly understood, roles
in chromosome organization and dynamics during meiosis. In Caenorhabditis
elegans, four such proteins (HIM-3, HTP-1, HTP-2, and HTP-3) have distinct but
overlapping functions. Through combined biochemical, structural, and in vivo
analysis, we find that these proteins form hierarchical complexes through
binding of their HORMA domains to cognate peptides within their partners'
C-terminal tails, analogous to the "safety belt" binding mechanism of
Mad2. These interactions are critical for recruitment of HIM-3, HTP-1, and HTP-2
to chromosome axes. HTP-3, in addition to recruiting the other HORMA domain
proteins to the axis, plays an independent role in sister chromatid cohesion and
double-strand break formation. Finally, we find that mammalian HORMAD1 binds a
motif found both at its own C terminus and at that of HORMAD2, indicating that
this mode of intermolecular association is a conserved feature of meiotic
chromosome structure in eukaryotes.
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