PDBsum entry 4txk

Oxidoreductase

PDB id: 4txk

Contents

Protein chain

581 a.a.

Ligands

PEG

FAD

Waters ×38

PDB id:

4txk

Name:

Oxidoreductase

Title:

Construct of mical-1 containing the monooxygenase and calponin homology domains

Structure:

Protein-methionine sulfoxide oxidase mical1. Chain: a. Fragment: unp resdiues 2-615. Synonym: molecule interacting with casl protein 1,mmical1,nedd9- interacting protein with calponin homology and lim domains. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: mical1, mical, nical. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.88Å R-factor: 0.206 R-free: 0.272

Authors:

S.S.Alqassim,L.M.Amzel,M.A.Bianchet

Key ref:

S.S.Alqassim et al. Structural insights into modulation of mical activity calponin homology (ch) domain. To be published, .

Date:

03-Jul-14 Release date: 23-Dec-15

Protein chain

Q8VDP3  (MICA1_MOUSE) -  [F-actin]-monooxygenase MICAL1 from Mus musculus

Seq: 1048 a.a.

Struc: 581 a.a.

Enzyme reactions

• Enzyme class 2: E.C.1.14.13.225 - F-actin monooxygenase.
• Reaction: L-methionyl-[F-actin] + NADPH + O2 + H⁺ = L-methionyl-(R)-S-oxide- [F-actin] + NADP⁺ + H2O

L-methionyl-[F-actin] + NADPH + O2 (Bound ligand (Het Group name = FAD) matches with 71.19% similarity) + H(+) = L-methionyl-(R)-S-oxide- [F-actin] + NADP(+) + H2O

• Enzyme class 3: E.C.1.6.3.1 - NAD(P)H oxidase (H2O2-forming).
• Reaction:
  1. NADPH + O2 + H⁺ = H2O2 + NADP⁺
  2. NADH + O2 + H⁺ = H2O2 + NAD⁺
• Cofactor: Ca(2+); FAD; Heme

Ca(2+) FAD (Bound ligand (Het Group name = FAD) corresponds exactly) Heme

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site