spacer
spacer

PDBsum entry 4tsv

Go to PDB code: 
Top Page protein links
Lymphokine PDB id
4tsv
Jmol
Contents
Protein chain
148 a.a. *
Waters ×70
* Residue conservation analysis

References listed in PDB file
Key reference
Title High resolution crystal structure of a human tumor necrosis factor-Alpha mutant with low systemic toxicity.
Authors S.S.Cha, J.S.Kim, H.S.Cho, N.K.Shin, W.Jeong, H.C.Shin, Y.J.Kim, J.H.Hahn, B.H.Oh.
Ref. J Biol Chem, 1998, 273, 2153-2160. [DOI no: 10.1074/jbc.273.4.2153]
PubMed id 9442056
Abstract
A human tumor necrosis factor-alpha (TNF-alpha) mutant (M3S) with low systemic toxicity in vivo was designed, and its structures in two different crystal packings were determined crystallographically at 1.8 and 2.15-A resolution, respectively, to explain altered biological activities of the mutant. M3S contains four changes: a hydrophilic substitution of L29S, two hydrophobic substitutions of S52I and Y56F, and a deletion of the N-terminal seven amino acids that is disordered in the structure of wild-type TNF-alpha. Compared with wild-type TNF-alpha, it exhibits 11- and 71-fold lower binding affinities for the human TNF-R55 and TNF-R75 receptors, respectively, and in vitro cytotoxic effect and in vivo systemic toxicity of M3S are 20 and 10 times lower, respectively. However, in a transplanted solid tumor mouse model, M3S suppresses tumor growth more efficiently than wild-type TNF-alpha. M3S is highly resistant to proteolysis by trypsin, and it exhibits increased thermal stability and a prolonged half-life in vivo. The L29S mutation causes substantial restructuring of the loop containing residues 29-36 into a rigid segment as a consequence of induced formation of intra- and intersubunit interactions, explaining the altered receptor binding affinity and thermal stability. A mass spectrometric analysis identified major proteolytic cleavage sites located on this loop, and thus the increased resistance of M3S to the proteolysis is consistent with the increased rigidity of the loop. The S52I and Y56F mutations do not induce a noticeable conformational change. The side chain of Phe56 projects into a hydrophobic cavity, while Ile52 is exposed to the bulk solvent. Ile52 should be involved in hydrophobic interactions with the receptors, since a mutant containing the same mutations as in M3S except for the L29S mutation exhibits an increased receptor binding affinity. The low systemic toxicity of M3S appears to be the effect of the reduced and selective binding affinities for the TNF receptors, and the superior tumor-suppression of M3S appears to be the effect of its weak but longer antitumoral activity in vivo compared with wild-type TNF-alpha. It is also expected that the 1.8-A resolution structure will serve as an accurate model for explaining the structure-function relationship of wild-type TNF-alpha and many TNF-alpha mutants reported previously and for the design of new TNF-alpha mutants.
Figure 3.
Fig. 3. A hydrogen-bonded network induced by the I29S mutation. White dotted lines indicate hydrogen bonds. Water molecules are in magenta. Oxygen and nitrogen atoms are in red and yellow, respectively. Each subunit is represented by green and pink, respectively. Only the side chains involved in the interactions are shown for clarity.
Figure 8.
Fig. 8. Stereo diagram of the interactions of the loop containing Arg44 and of the loop containing Arg31 and Arg32. Arginine residues are in magenta, and hydrophobic side chains are in green. White dotted lines indicate hydrogen bonds. Oxygen and nitrogen atoms are in red and yellow, respectively. Only the^ side chains involved in the interactions are shown for clarity.
The above figures are reprinted by permission from the ASBMB: J Biol Chem (1998, 273, 2153-2160) copyright 1998.
Secondary reference #1
Title The structure of tumor necrosis factor-Alpha at 2.6 a resolution. Implications for receptor binding.
Authors M.J.Eck, S.R.Sprang.
Ref. J Biol Chem, 1989, 264, 17595-17605.
PubMed id 2551905
Abstract
PROCHECK
Go to PROCHECK summary
 Headers