PDBsum entry 4rvc

Transport protein

PDB id: 4rvc

Contents

Protein chain

240 a.a.

Waters ×206

PDB id:

4rvc

Name:

Transport protein

Title:

Structure of atp binding subunit of abc transporter

Structure:

Abc transporter atp-binding protein. Chain: a. Fragment: unp residues 13-257. Engineered: yes

Source:

Geobacillus kaustophilus. Organism_taxid: 1337888. Strain: gblys. Gene: gbl_2030. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.77Å R-factor: 0.216 R-free: 0.250

Authors:

M.Manjula,K.J.Pampa,N.K.Lokanath

Key ref:

M.Manjula et al. (2015). Crystal structure of ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus. Biochem Biophys Res Commun, 459, 113-117. PubMed id: 25724946 DOI: 10.1016/j.bbrc.2015.02.079

Date:

26-Nov-14 Release date: 18-Mar-15

Protein chain

U2YAF6  (U2YAF6_GEOKU) -  ABC transporter ATP-binding protein from Geobacillus kaustophilus GBlys

Seq: 257 a.a.

Struc: 240 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1016/j.bbrc.2015.02.079

Biochem Biophys Res Commun 459:113-117 (2015)

PubMed id: 25724946

Crystal structure of ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus.

M.Manjula, K.J.Pampa, S.M.Kumar, S.Mukherjee, N.Kunishima, K.S.Rangappa, N.K.Lokanath.

ABSTRACT

The ATP binding cassette (ABC) transporters, represent one of the largest superfamilies of primary transporters, which are very essential for various biological functions. The crystal structure of ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus has been determined at 1.77 Å resolution. The crystal structure revealed that the protomer has two thick arms, (arm I and II), which resemble 'L' shape. The ATP-binding pocket is located close to the end of arm I. ATP molecule is docked into the active site of the protein. The dimeric crystal structure of ATP-binding subunit of ABC transporter from G. kaustophilus has been compared with the previously reported crystal structure of ATP-binding subunit of ABC transporter from Salmonella typhimurium.