PDBsum entry 4rg6

Protein binding

PDB id: 4rg6

Contents

Protein chains

474 a.a.

35 a.a.

PDB id:

4rg6

Name:

Protein binding

Title:

Crystal structure of apc3-apc16 complex

Structure:

Cell division cycle protein 27 homolog. Chain: a, b. Fragment: unp residues 1-180, 447-824. Synonym: anaphase-promoting complex subunit 3, apc3, cdc27 homolog, cdc27hs, h-nuc. Engineered: yes. Anaphase-promoting complex subunit 16. Chain: s. Fragment: unp residues 74-109.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cdc27, anapc3, d0s1430e, d17s978e. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: anapc16, c10orf104. Expression_system_taxid: 7108

Resolution:

3.30Å R-factor: 0.205 R-free: 0.242

Authors:

M.Yamaguchi,S.Yu,D.J.Miller,B.A.Schulman

Key ref:

M.Yamaguchi et al. (2015). Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome. J Mol Biol, 427, 1748-1764. PubMed id: 25490258 DOI: 10.1016/j.jmb.2014.11.020

Date:

29-Sep-14 Release date: 24-Dec-14

Protein chains

P30260  (CDC27_HUMAN) -  Cell division cycle protein 27 homolog from Homo sapiens

Seq: 824 a.a.

Struc: 474 a.a.

Protein chain

Q96DE5  (APC16_HUMAN) -  Anaphase-promoting complex subunit 16 from Homo sapiens

Seq: 110 a.a.

Struc: 35 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/j.jmb.2014.11.020

J Mol Biol 427:1748-1764 (2015)

PubMed id: 25490258

Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome.

M.Yamaguchi, S.Yu, R.Qiao, F.Weissmann, D.J.Miller, R.VanderLinden, N.G.Brown, J.J.Frye, J.M.Peters, B.A.Schulman.

ABSTRACT

The anaphase-promoting complex/cyclosome (APC/C) is a massive E3 ligase that controls mitosis by catalyzing ubiquitination of key cell cycle regulatory proteins. The APC/C assembly contains two subcomplexes: the "Platform" centers around a cullin-RING-like E3 ligase catalytic core; the "Arc Lamp" is a hub that mediates transient association with regulators and ubiquitination substrates. The Arc Lamp contains the small subunits APC16, CDC26, and APC13, and tetratricopeptide repeat (TPR) proteins (APC7, APC3, APC6, and APC8) that homodimerize and stack with quasi-2-fold symmetry. Within the APC/C complex, APC3 serves as center for regulation. APC3's TPR motifs recruit substrate-binding coactivators, CDC20 and CDH1, via their C-terminal conserved Ile-Arg (IR) tail sequences. Human APC3 also binds APC16 and APC7 and contains a >200-residue loop that is heavily phosphorylated during mitosis, although the basis for APC3 interactions and whether loop phosphorylation is required for ubiquitination are unclear. Here, we map the basis for human APC3 assembly with APC16 and APC7, report crystal structures of APC3Δloop alone and in complex with the C-terminal domain of APC16, and test roles of APC3's loop and IR tail binding surfaces in APC/C-catalyzed ubiquitination. The structures show how one APC16 binds asymmetrically to the symmetric APC3 dimer and, together with biochemistry and prior data, explain how APC16 recruits APC7 to APC3, show how APC3's C-terminal domain is rearranged in the full APC/C assembly, and visualize residues in the IR tail binding cleft important for coactivator-dependent ubiquitination. Overall, the results provide insights into assembly, regulation, and interactions of TPR proteins and the APC/C.