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PDBsum entry 4rex
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Protein binding
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PDB id
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4rex
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the first ww domain of human yap2 isoform.
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Authors
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S.Martinez-Rodriguez,
J.Bacarizo,
I.Luque,
A.Camara-Artigas.
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Ref.
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J Struct Biol, 2015,
191,
381-387.
[DOI no: ]
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PubMed id
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Abstract
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The WW domains are the smallest modular domains known. The study of the
structural basis of their stability is important to understand their
physiological role. These domains are intrinsically flexible, which makes them
difficult to crystallize. The first WW domain of the human Yes tyrosine kinase
Associated Protein (YAP) has been crystallized and its structure has been solved
by X-ray diffraction at 1.6Å resolution. Crystals belong to the orthorhombic
space group P21212 with unit cell parameters a=42.67, b=43.10 and c=21.30. The
addition of proline and other small-molecule additives improves drastically the
quality of the crystals. The interactions that stabilize this minimal modular
domain have been analysed. This crystal structure reveals that, besides the
stabilization of the hydrophobic core of the protein by the aromatic cluster
formed by Trp177-Phe189-Pro202, some salt-bridges interactions might affect the
stability of the domain.
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