PDBsum entry 4r9u

Hydrolase

PDB id: 4r9u

Contents

Protein chains

324 a.a.

248 a.a.

Ligands

LDA ×4

ANP ×2

Metals

_MG ×2

References listed in PDB file

Key reference

• Title: Structure of AMP-Pnp-Bound btucd and mechanism of ATP-Powered vitamin b12 transport by btucd-F.
• Authors: V.M.Korkhov, S.A.Mireku, D.B.Veprintsev, K.P.Locher.
• Ref.: Nat Struct Biol, 2014, 21, 1097-1099. [DOI no: 10.1038/nsmb.2918]
• PubMed id: 25402482

Abstract

The reaction mechanism of BtuCD-F-catalyzed vitamin B12 transport into Escherichia coli is currently unclear. Here we present the structure of the last missing state in the form of AMP-PNP-bound BtuCD, trapped by a disulfide cross-link. Our structural and biochemical data allow a consistent mechanism to be formulated, thus rationalizing the roles of substrate, ATP and substrate-binding protein.