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PDBsum entry 4r6g
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De novo protein
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PDB id
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4r6g
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References listed in PDB file
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Key reference
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Title
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Control of repeat-Protein curvature by computational protein design.
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Authors
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K.Park,
B.W.Shen,
F.Parmeggiani,
P.S.Huang,
B.L.Stoddard,
D.Baker.
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Ref.
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Nat Struct Biol, 2015,
22,
167-174.
[DOI no: ]
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PubMed id
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Abstract
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Shape complementarity is an important component of molecular recognition, and
the ability to precisely adjust the shape of a binding scaffold to match a
target of interest would greatly facilitate the creation of high-affinity
protein reagents and therapeutics. Here we describe a general approach to
control the shape of the binding surface on repeat-protein scaffolds and apply
it to leucine-rich-repeat proteins. First, self-compatible building-block
modules are designed that, when polymerized, generate surfaces with unique but
constant curvatures. Second, a set of junction modules that connect the
different building blocks are designed. Finally, new proteins with
custom-designed shapes are generated by appropriately combining building-block
and junction modules. Crystal structures of the designs illustrate the power of
the approach in controlling repeat-protein curvature.
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