PDBsum entry 4r09

Immune system

PDB id: 4r09

Contents

Protein chains

749 a.a.

Ligands

NAG-NAG-BMA-MAN-MAN ×2

NAG-NAG ×6

NAG-NAG-BMA ×3

URI ×4

06S ×4

UPT ×4

NAG ×3

PDB id:

4r09

Name:

Immune system

Title:

Crystal structure of human tlr8 in complex with orn06s

Structure:

Toll-like receptor 8. Chain: a, b, c, d. Fragment: extracellular domain. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tlr8, unq249/pro286. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider 2(s2) cells.

Resolution:

2.62Å R-factor: 0.220 R-free: 0.285

Authors:

H.Tanji,U.Ohto,T.Shimizu

Key ref:

H.Tanji et al. (2015). Toll-like receptor 8 senses degradation products of single-stranded RNA. Nat Struct Biol, 22, 109-115. PubMed id: 25599397 DOI: 10.1038/nsmb.2943

Date:

30-Jul-14 Release date: 14-Jan-15

Protein chains

Q9NR97  (TLR8_HUMAN) -  Toll-like receptor 8 from Homo sapiens

Seq: 1041 a.a.

Struc: 749 a.a.

DOI no: 10.1038/nsmb.2943

Nat Struct Biol 22:109-115 (2015)

PubMed id: 25599397

Toll-like receptor 8 senses degradation products of single-stranded RNA.

H.Tanji, U.Ohto, T.Shibata, M.Taoka, Y.Yamauchi, T.Isobe, K.Miyake, T.Shimizu.

ABSTRACT

Toll-like receptor 8 (TLR8) recognizes viral or bacterial single-stranded RNA (ssRNA) and activates innate immune systems. TLR8 is activated by uridine- and guanosine-rich ssRNA as well as by certain synthetic chemicals; however, the molecular basis for ssRNA recognition has remained unknown. In this study, to elucidate the recognition mechanism of ssRNA, we determined the crystal structures of human TLR8 in complex with ssRNA. TLR8 recognized two degradation products of ssRNA-uridine and a short oligonucleotide-at two distinct sites: uridine bound the site on the dimerization interface where small chemical ligands are recognized, whereas short oligonucleotides bound a newly identified site on the concave surface of the TLR8 horseshoe structure. Site-directed mutagenesis revealed that both binding sites were essential for activation of TLR8 by ssRNA. These results demonstrate that TLR8 is a sensor for both uridine and a short oligonucleotide derived from RNA.