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PDBsum entry 4r07
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Immune system
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PDB id
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4r07
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PDB id:
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| Name: |
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Immune system
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Title:
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Crystal structure of human tlr8 in complex with orn06
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Structure:
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Toll-like receptor 8. Chain: a, b, c, d. Fragment: extracellular domain. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: tlr8, unq249/pro286. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider 2(s2) cells.
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Resolution:
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2.00Å
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R-factor:
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0.190
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R-free:
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0.234
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Authors:
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H.Tanji,U.Ohto,T.Shibata,M.Taoka,Y.Yamauchi,T.Isobe,K.Miyake, T.Shimizu
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Key ref:
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H.Tanji
et al.
(2015).
Toll-like receptor 8 senses degradation products of single-stranded RNA.
Nat Struct Biol,
22,
109-115.
PubMed id:
DOI:
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Date:
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30-Jul-14
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Release date:
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14-Jan-15
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PROCHECK
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Headers
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References
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Q9NR97
(TLR8_HUMAN) -
Toll-like receptor 8 from Homo sapiens
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Seq:
Struc:
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1041 a.a.
747 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Nat Struct Biol
22:109-115
(2015)
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PubMed id:
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Toll-like receptor 8 senses degradation products of single-stranded RNA.
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H.Tanji,
U.Ohto,
T.Shibata,
M.Taoka,
Y.Yamauchi,
T.Isobe,
K.Miyake,
T.Shimizu.
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ABSTRACT
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Toll-like receptor 8 (TLR8) recognizes viral or bacterial single-stranded RNA
(ssRNA) and activates innate immune systems. TLR8 is activated by uridine- and
guanosine-rich ssRNA as well as by certain synthetic chemicals; however, the
molecular basis for ssRNA recognition has remained unknown. In this study, to
elucidate the recognition mechanism of ssRNA, we determined the crystal
structures of human TLR8 in complex with ssRNA. TLR8 recognized two degradation
products of ssRNA-uridine and a short oligonucleotide-at two distinct sites:
uridine bound the site on the dimerization interface where small chemical
ligands are recognized, whereas short oligonucleotides bound a newly identified
site on the concave surface of the TLR8 horseshoe structure. Site-directed
mutagenesis revealed that both binding sites were essential for activation of
TLR8 by ssRNA. These results demonstrate that TLR8 is a sensor for both uridine
and a short oligonucleotide derived from RNA.
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Links
