 |
PDBsum entry 4qrr
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Immune system
|
PDB id
|
|
|
|
4qrr
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
276 a.a.
|
|
|
|
|
|
|
|
|
|
|
99 a.a.
|
|
|
|
|
|
|
|
|
|
|
203 a.a.
|
|
|
|
|
|
|
|
|
|
|
241 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Immune system
|
|
|
Title:
|
|
Crystal structure of hla b 3501-Ips in complex with a delta-beta tcr, clone 12 tcr
|
|
Structure:
|
|
Hla class i histocompatibility antigen, b-35 alpha chain. Chain: a. Synonym: mhc class i antigen b 35. Engineered: yes. Beta-2-microglobulin. Chain: b. Synonym: beta-2-microglobulin form pi 5.3. Engineered: yes. Clone12 tcr beta chain.
|
|
Source:
|
|
Homo sapiens. Human. Organism_taxid: 9606. Gene: hla-b, hlab. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: b2m, cdabp0092, hdcma22p. Synthetic: yes. Human herpesvirus 5.
|
|
Resolution:
|
|
|
3.00Å
|
R-factor:
|
0.213
|
R-free:
|
0.258
|
|
|
Authors:
|
|
S.Gras,E.Chabrol,J.Rossjohn
|
|
Key ref:
|
|
D.G.Pellicci
et al.
(2014).
The molecular bases of δ/αβ T cell-mediated antigen recognition.
J Exp Med,
211,
2599-2615.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
02-Jul-14
|
Release date:
|
10-Dec-14
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P01889
(1B07_HUMAN) -
HLA class I histocompatibility antigen, B alpha chain from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
362 a.a.
276 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
P61769
(B2MG_HUMAN) -
Beta-2-microglobulin from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
119 a.a.
99 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
J Exp Med
211:2599-2615
(2014)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
The molecular bases of δ/αβ T cell-mediated antigen recognition.
|
|
D.G.Pellicci,
A.P.Uldrich,
J.Le Nours,
F.Ross,
E.Chabrol,
S.B.Eckle,
R.de Boer,
R.T.Lim,
K.McPherson,
G.Besra,
A.R.Howell,
L.Moretta,
J.McCluskey,
M.H.Heemskerk,
S.Gras,
J.Rossjohn,
D.I.Godfrey.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
αβ and γδ T cells are disparate T cell lineages that can respond to distinct
antigens (Ags) via the use of the αβ and γδ T cell Ag receptors (TCRs),
respectively. Here we characterize a population of human T cells, which we term
δ/αβ T cells, expressing TCRs comprised of a TCR-δ variable gene (Vδ1)
fused to joining α and constant α domains, paired with an array of TCR-β
chains. We demonstrate that these cells, which represent ∼50% of all Vδ1(+)
human T cells, can recognize peptide- and lipid-based Ags presented by human
leukocyte antigen (HLA) and CD1d, respectively. Similar to type I natural killer
T (NKT) cells, CD1d-lipid Ag-reactive δ/αβ T cells recognized
α-galactosylceramide (α-GalCer); however, their fine specificity for other
lipid Ags presented by CD1d, such as α-glucosylceramide, was distinct from type
I NKT cells. Thus, δ/αβTCRs contribute new patterns of Ag specificity to the
human immune system. Furthermore, we provide the molecular bases of how
δ/αβTCRs bind to their targets, with the Vδ1-encoded region providing a
major contribution to δ/αβTCR binding. Our findings highlight how components
from αβ and γδTCR gene loci can recombine to confer Ag specificity, thus
expanding our understanding of T cell biology and TCR diversity.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
| | |
Links
