 |
PDBsum entry 4qqz
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Hydrolase/DNA
|
PDB id
|
|
|
|
4qqz
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase/DNA
|
|
|
Title:
|
|
Crystal structure of t. Fusca cas3-amppnp
|
|
Structure:
|
|
Crispr-associated helicase, cas3 family. Chain: a, c, e, g. Engineered: yes. DNA (5'-d(p Ap Ap Ap Ap Ap Ap Ap Ap Ap Ap Ap A)-3'). Chain: b, d, f, h. Engineered: yes
|
|
Source:
|
|
Thermobifida fusca. Organism_taxid: 269800. Strain: yx. Gene: tfu_1593. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
|
|
Resolution:
|
|
|
2.93Å
|
R-factor:
|
0.183
|
R-free:
|
0.234
|
|
|
Authors:
|
|
A.Ke,Y.Huo,K.H.Nam
|
|
Key ref:
|
|
Y.Huo
et al.
(2014).
Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation.
Nat Struct Biol,
21,
771-777.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
30-Jun-14
|
Release date:
|
27-Aug-14
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q47PJ0
(Q47PJ0_THEFY) -
CRISPR-associated helicase, Cas3 family from Thermobifida fusca (strain YX)
|
|
|
|
Seq:
Struc:
|
|
|
|
944 a.a.
903 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
A-A-A-A-A-A-A-A-A-A-A
11 bases
|
|
|
|
A-A-A-A-A-A-A-A-A-A-A
11 bases
|
|
|
|
A-A-A-A-A-A-A-A-A-A-A
11 bases
|
|
|
|
A-A-A-A-A-A-A-A-A-A-A
11 bases
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Nat Struct Biol
21:771-777
(2014)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation.
|
|
Y.Huo,
K.H.Nam,
F.Ding,
H.Lee,
L.Wu,
Y.Xiao,
M.D.Farchione,
S.Zhou,
K.Rajashankar,
I.Kurinov,
R.Zhang,
A.Ke.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
