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PDBsum entry 4pqz
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Transcription
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PDB id
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4pqz
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References listed in PDB file
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Key reference
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Title
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High-Resolution crystal structure reveals a hepn domain at the c-Terminal region of s. Cerevisiae RNA endonuclease swt1.
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Authors
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S.Peng,
K.Zhou,
W.Wang,
Z.Gao,
Y.Dong,
Q.Liu.
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Ref.
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Biochem Biophys Res Commun, 2014,
453,
826-832.
[DOI no: ]
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PubMed id
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Abstract
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Swt1 is an RNA endonuclease that plays an important role in quality control of
nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however,
its structural details remain to be elucidated. Here, we report the crystal
structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae,
which shares common characteristics of higher eukaryotes and prokaryotes
nucleotide binding (HEPN) domain superfamily. To study in detail the full-length
protein structure, we analyzed the low-resolution architecture of Swt1 in
solution using small angle X-ray scattering (SAXS) method. Both the CT domain
and middle domain exhibited a good fit upon superimposing onto the molecular
envelope of Swt1. Our study provides the necessary structural information for
detailed analysis of the functional role of Swt1, and its importance in the
process of nuclear mRNP surveillance.
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