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PDBsum entry 4pog
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Replication, DNA binding protein/DNA
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PDB id
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4pog
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References listed in PDB file
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Key reference
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Title
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A conserved mcm single-Stranded DNA binding element is essential for replication initiation.
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Authors
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C.A.Froelich,
S.Kang,
L.B.Epling,
S.P.Bell,
E.J.Enemark.
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Ref.
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Elife, 2014,
3,
e01993.
[DOI no: ]
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PubMed id
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Abstract
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The ring-shaped MCM helicase is essential to all phases of DNA replication. The
complex loads at replication origins as an inactive double-hexamer encircling
duplex DNA. Helicase activation converts this species to two active single
hexamers that encircle single-stranded DNA (ssDNA). The molecular details of MCM
DNA interactions during these events are unknown. We determined the crystal
structure of the Pyrococcus furiosus MCM N-terminal domain hexamer bound to
ssDNA and define a conserved MCM-ssDNA binding motif (MSSB). Intriguingly, ssDNA
binds the MCM ring interior perpendicular to the central channel with defined
polarity. In eukaryotes, the MSSB is conserved in several Mcm2-7 subunits, and
MSSB mutant combinations in S. cerevisiae Mcm2-7 are not viable. Mutant Mcm2-7
complexes assemble and are recruited to replication origins, but are defective
in helicase loading and activation. Our findings identify an important MCM-ssDNA
interaction and suggest it functions during helicase activation to select the
strand for translocation. DOI: http://dx.doi.org/10.7554/eLife.01993.001.
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