|
|
||
|
|
|
|
|
UniProt functional annotation for P36923 | ||
|
|
|
|
|
|
||
| UniProt code: P36923. |
|
||
| Organism: | |
Enterococcus faecalis (strain ATCC 700802 / V583). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus. |
|
||
|
||
| Function: | |
Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids (AroAA). Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsylon-amino group of Lys-170 at the active site. {ECO:0000269|PubMed:25072253}. |
|
||
| Catalytic activity: | |
Reaction=3-dehydroquinate = 3-dehydroshikimate + H2O; Xref=Rhea:RHEA:21096, ChEBI:CHEBI:15377, ChEBI:CHEBI:16630, ChEBI:CHEBI:32364; EC=4.2.1.10; Evidence={ECO:0000255|HAMAP- Rule:MF_00214, ECO:0000269|PubMed:25072253}; |
| Activity regulation: | |
Inhibited by flavonoids such as datiscetin, naringenin, marein and phloretin. {ECO:0000269|PubMed:25072253}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=65 uM for 3-dehydroquinate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:25072253}; Note=Kcat is 110 sec(-1) for dehydratase activity with 3- dehydroquinate (at pH 7.5 and 25 degrees Celsius). {ECO:0000269|PubMed:25072253}; |
| Pathway: | |
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. {ECO:0000255|HAMAP-Rule:MF_00214}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_00214, ECO:0000269|PubMed:25072253}. |
| Similarity: | |
Belongs to the type-I 3-dehydroquinase family. {ECO:0000255|HAMAP-Rule:MF_00214}. |
Annotations taken from UniProtKB at the EBI.