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PDBsum entry 4ph0
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Viral protein
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PDB id
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4ph0
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References listed in PDB file
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Key reference
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Title
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Structural virology. Conformational plasticity of a native retroviral capsid revealed by X-Ray crystallography.
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Authors
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G.Obal,
F.Trajtenberg,
F.Carrión,
L.Tomé,
N.Larrieux,
X.Zhang,
O.Pritsch,
A.Buschiazzo.
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Ref.
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Science, 2015,
349,
95-98.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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Retroviruses depend on self-assembly of their capsid proteins (core particle) to
yield infectious mature virions. Despite the essential role of the retroviral
core, its high polymorphism has hindered high-resolution structural analyses.
Here, we report the x-ray structure of the native capsid (CA) protein from
bovine leukemia virus. CA is organized as hexamers that deviate substantially
from sixfold symmetry, yet adjust to make two-dimensional pseudohexagonal arrays
that mimic mature retroviral cores. Intra- and interhexameric quasi-equivalent
contacts are uncovered, with flexible trimeric lateral contacts among hexamers,
yet preserving very similar dimeric interfaces making the lattice. The
conformation of each capsid subunit in the hexamer is therefore dictated by
long-range interactions, revealing how the hexamers can also assemble into
closed core particles, a relevant feature of retrovirus biology.
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