PDBsum entry 4p7i

Signaling protein/protein binding

PDB id: 4p7i

Contents

Protein chains

294 a.a.

15 a.a.

Ligands

ASP-ILE-ILE-LEU-SER-LEU-ASN

GOL

Waters ×31

PDB id:

4p7i

Name:

Signaling protein/protein binding

Title:

Crystal structure of the merlin ferm/dcaf1 complex

Structure:

Merlin. Chain: a, b. Fragment: ferm domain residues 1-313. Synonym: moesin-ezrin-radixin-like protein,neurofibromin-2, schwannomin. Engineered: yes. Protein vprbp. Chain: c, d. Fragment: unp residues 998-1058.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: nf2, nf-2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Homo sapiens. Human. Organism_taxid: 9606.

Resolution:

2.60Å R-factor: 0.226 R-free: 0.256

Authors:

Z.Wei,Y.Li,M.Zhang

Key ref:

Y.Li et al. (2014). Structural basis of the binding of Merlin FERM domain to the E3 ubiquitin ligase substrate adaptor DCAF1. J Biol Chem, 289, 14674-14681. PubMed id: 24706749 DOI: 10.1074/jbc.M114.551184

Date:

27-Mar-14 Release date: 09-Apr-14

Protein chains

P46662  (MERL_MOUSE) -  Merlin from Mus musculus

Seq: 596 a.a.

Struc: 294 a.a.

Protein chain

Q9Y4B6  (DCAF1_HUMAN) -  DDB1- and CUL4-associated factor 1 from Homo sapiens

Seq: 1507 a.a.

Struc: 15 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain C: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1074/jbc.M114.551184

J Biol Chem 289:14674-14681 (2014)

PubMed id: 24706749

Structural basis of the binding of Merlin FERM domain to the E3 ubiquitin ligase substrate adaptor DCAF1.

Y.Li, Z.Wei, J.Zhang, Z.Yang, M.Zhang.

ABSTRACT

No abstract given.