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PDBsum entry 4p1w
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Protein transport
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PDB id
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4p1w
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Contents |
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72 a.a.
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135 a.a.
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399 a.a.
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66 a.a.
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126 a.a.
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PDB id:
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| Name: |
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Protein transport
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Title:
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Crystal structure of atg13(17br)-atg17-atg29-atg31 complex
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Structure:
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Atg29. Chain: a, d. Fragment: unp residues 1-86. Synonym: klth0c07942p. Engineered: yes. Atg31. Chain: b, e. Synonym: klth0d11660p. Engineered: yes.
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Source:
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Lachancea thermotolerans. Yeast. Organism_taxid: 559295. Strain: atcc 56472 / cbs 6340 / nrrl y-8284. Gene: klth0d11660g. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: klth0c07942g. Gene: klth0d15642g.
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Resolution:
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3.20Å
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R-factor:
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0.261
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R-free:
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0.293
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Authors:
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Y.Fujioka,N.N.Noda
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Key ref:
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Y.Fujioka
et al.
(2014).
Structural basis of starvation-induced assembly of the autophagy initiation complex.
Nat Struct Biol,
21,
513-521.
PubMed id:
DOI:
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Date:
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27-Feb-14
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Release date:
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07-May-14
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PROCHECK
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Headers
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References
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C5DF24
(C5DF24_LACTC) -
Autophagy-related protein 29 from Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
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Seq:
Struc:
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171 a.a.
72 a.a.*
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C5DEB9
(C5DEB9_LACTC) -
KLTH0C07942p from Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
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Seq:
Struc:
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145 a.a.
135 a.a.
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C5DFJ6
(C5DFJ6_LACTC) -
Autophagy-related protein 17 from Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
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Seq:
Struc:
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413 a.a.
399 a.a.
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DOI no:
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Nat Struct Biol
21:513-521
(2014)
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PubMed id:
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Structural basis of starvation-induced assembly of the autophagy initiation complex.
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Y.Fujioka,
S.W.Suzuki,
H.Yamamoto,
C.Kondo-Kakuta,
Y.Kimura,
H.Hirano,
R.Akada,
F.Inagaki,
Y.Ohsumi,
N.N.Noda.
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ABSTRACT
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Assembly of the preautophagosomal structure (PAS) is essential for autophagy
initiation in yeast. Starvation-induced dephosphorylation of Atg13 is required
for the formation of the Atg1-Atg13-Atg17-Atg29-Atg31 complex (Atg1 complex), a
prerequisite for PAS assembly. However, molecular details underlying these
events have not been established. Here we studied the interactions of yeast
Atg13 with Atg1 and Atg17 by X-ray crystallography. Atg13 binds tandem
microtubule interacting and transport domains in Atg1, using an elongated
helix-loop-helix region. Atg13 also binds Atg17, using a short region, thereby
bridging Atg1 and Atg17 and leading to Atg1-complex formation. Dephosphorylation
of specific serines in Atg13 enhanced its interaction with not only Atg1 but
also Atg17. These observations update the autophagy-initiation model as follows:
upon starvation, dephosphorylated Atg13 binds both Atg1 and Atg17, and this
promotes PAS assembly and autophagy progression.
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