PDBsum entry 4ozy

Isomerase

PDB id: 4ozy

Contents

Protein chain

423 a.a.

PDB id:

4ozy

Name:

Isomerase

Title:

Crystal structure of the periplasmic alginate epimerase algg t265n mutant

Structure:

Poly(beta-d-mannuronate) c5 epimerase. Chain: a. Fragment: unp residues 69 to 491. Engineered: yes. Mutation: yes

Source:

Pseudomonas syringae pv. Tomato. Organism_taxid: 223283. Strain: dc3000. Gene: algg, pspto_1238. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.90Å R-factor: 0.180 R-free: 0.221

Authors:

P.L.Howell,F.Wolfram,H.Robinson

Key ref:

P.L.Howell et al. Crystal structure of the periplasmic alginate epimera t265n mutant. To be published, .

Date:

19-Feb-14 Release date: 04-Mar-15

Protein chain

Q887Q3  (ALGG_PSESM) -  Mannuronan C5-epimerase from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

Seq: 536 a.a.

Struc: 423 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.5.1.3.37 - mannuronan 5-epimerase.
• Reaction: [(1->4)-beta-D-mannuronosyl](n) = [alginate](n)