PDBsum entry 4ora

Hydrolase/metal binding protein

PDB id: 4ora

Contents

Protein chains

397 a.a.

166 a.a.

Metals

_FE

_CA ×4

_ZN

Waters ×45

PDB id:

4ora

Name:

Hydrolase/metal binding protein

Title:

Crystal structure of a human calcineurin mutant

Structure:

Serine/threonine-protein phosphatase 2b catalytic subunit beta isoform. Chain: a. Synonym: cam-prp catalytic subunit, calmodulin-dependent calcineurin a subunit beta isoform. Engineered: yes. Mutation: yes. Calcineurin subunit b type 1. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: calna2, calnb, cna2, ppp3cb. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cna2, cnb, ppp3r1.

Resolution:

2.75Å R-factor: 0.202 R-free: 0.249

Authors:

S.J.Li,J.Wang,J.W.Wu,Z.X.Wang

Key ref:

S.J.Li et al. Cooperative autoinhibition and multi-Level activation mechanisms of calcineurin. To be published, . PubMed id: 26794871

Date:

11-Feb-14 Release date: 20-May-15

Protein chain

P16298  (PP2BB_HUMAN) -  Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform from Homo sapiens

Seq: 524 a.a.

Struc: 397 a.a.*

Protein chain

P63098  (CANB1_HUMAN) -  Calcineurin subunit B type 1 from Homo sapiens

Seq: 170 a.a.

Struc: 166 a.a.

* PDB and UniProt seqs differ at 23 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site