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UniProt functional annotation for A0R3F9 | ||
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| UniProt code: A0R3F9. |
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| Organism: | |
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium. |
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| Function: | |
Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS) and of the universal stress protein (USP) MSMEG_4207. Acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes. {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103}. |
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| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| Activity regulation: | |
Allosterically regulated by cAMP. {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.1 uM for acetyl-CoA (with ACS as substrate) {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103}; KM=10 uM for acetyl-CoA (with USP as substrate) {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103}; KM=10.2 uM for ACS {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103}; KM=335 uM for USP {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:20507997}. |
| Disruption phenotype: | |
Disruption of this gene abolishes acetylation of USP. {ECO:0000269|PubMed:20507997}. |
Annotations taken from UniProtKB at the EBI.