PDBsum entry 4oi8

Transport protein,signaling protein/DNA

PDB id: 4oi8

Contents

Protein chains

211 a.a.

DNA/RNA

Waters ×25

PDB id:

4oi8

Name:

Transport protein,signaling protein/DNA

Title:

Rage is a nucleic acid receptor that promotes inflammatory responses to DNA.

Structure:

Advanced glycosylation end product-specific receptor. Chain: a, b. Fragment: unp residues 23-237. Synonym: rage, receptor for advanced glycosylation end products. Engineered: yes. 5'-d( Cp Cp Ap Tp Gp Ap Cp Tp Gp Tp Ap Gp Gp Ap Ap Ap Cp Tp Cp Tp Ap Gp A)-3'. Chain: e. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ager, rage. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

3.10Å R-factor: 0.229 R-free: 0.267

Authors:

T.Jin,J.Jiang,T.Xiao

Key ref:

C.M.Sirois et al. (2013). RAGE is a nucleic acid receptor that promotes inflammatory responses to DNA. J Exp Med, 210, 2447-2463. PubMed id: 24081950 DOI: 10.1084/jem.20120201

Date:

19-Jan-14 Release date: 30-Apr-14

Supersedes:

3s59

Protein chains

Q15109  (RAGE_HUMAN) -  Advanced glycosylation end product-specific receptor from Homo sapiens

Seq: 404 a.a.

Struc: 211 a.a.

DNA/RNA chains

C-C-A-T-G-A-C-T-G-T-A-G-G-A-A-A-C-T-C-T-A-G-A 23 bases

C-T-C-T-A-G-A-G-T-T-T-C-C-T-A-C-A-G-T-C-A-T-G 23 bases

DOI no: 10.1084/jem.20120201

J Exp Med 210:2447-2463 (2013)

PubMed id: 24081950

RAGE is a nucleic acid receptor that promotes inflammatory responses to DNA.

C.M.Sirois, T.Jin, A.L.Miller, D.Bertheloot, H.Nakamura, G.L.Horvath, A.Mian, J.Jiang, J.Schrum, L.Bossaller, K.Pelka, N.Garbi, Y.Brewah, J.Tian, C.Chang, P.S.Chowdhury, G.P.Sims, R.Kolbeck, A.J.Coyle, A.A.Humbles, T.S.Xiao, E.Latz.

ABSTRACT

Recognition of DNA and RNA molecules derived from pathogens or self-antigen is one way the mammalian immune system senses infection and tissue damage. Activation of immune signaling receptors by nucleic acids is controlled by limiting the access of DNA and RNA to intracellular receptors, but the mechanisms by which endosome-resident receptors encounter nucleic acids from the extracellular space are largely undefined. In this study, we show that the receptor for advanced glycation end-products (RAGE) promoted DNA uptake into endosomes and lowered the immune recognition threshold for the activation of Toll-like receptor 9, the principal DNA-recognizing transmembrane signaling receptor. Structural analysis of RAGE-DNA complexes indicated that DNA interacted with dimers of the outermost RAGE extracellular domains, and could induce formation of higher-order receptor complexes. Furthermore, mice deficient in RAGE were unable to mount a typical inflammatory response to DNA in the lung, indicating that RAGE is important for the detection of nucleic acids in vivo.