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UniProt functional annotation for Q9M8R4 | ||
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| UniProt code: Q9M8R4. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Possesses glyoxalase I activity. Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S- lactoylglutathione. May be involved in oxidative stress response. {ECO:0000269|PubMed:23651081}. |
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| Catalytic activity: | |
Reaction=(R)-S-lactoylglutathione = glutathione + methylglyoxal; Xref=Rhea:RHEA:19069, ChEBI:CHEBI:17158, ChEBI:CHEBI:57474, ChEBI:CHEBI:57925; EC=4.4.1.5; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.1 mM for methylglyoxal {ECO:0000269|PubMed:23651081}; KM=0.27 mM for glutathione {ECO:0000269|PubMed:23651081}; Note=kcat is 1700 min(-1) with methylglyoxal as substrate. kcat is 2200 min(-1) with glutathione as substrate.; pH dependence: Optimum pH is 6.8. {ECO:0000269|PubMed:23651081}; Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|PubMed:23651081}; |
| Subunit: | |
Homotrimer. {ECO:0000305|PubMed:23651081}. |
| Ptm: | |
Cys-120 and Cys-313 are oxidized to sulfinic acid. {ECO:0000269|PubMed:22232184}. |
| Similarity: | |
Belongs to the peptidase C56 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.