PDBsum entry 4ofv

Protein binding

PDB id: 4ofv

Contents

Protein chains

211 a.a.

PDB id:

4ofv

Name:

Protein binding

Title:

Refinement of rage-DNA complex in 3s58 without DNA

Structure:

Advanced glycosylation end product-specific receptor. Chain: a, b. Fragment: vc1 fragment. Synonym: receptor for advanced glycosylation end products. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ager, rage. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

3.10Å R-factor: 0.221 R-free: 0.240

Authors:

L.Yatime,G.R.Andersen

Key ref:

L.Yatime and G.R.Andersen (2014). The specificity of DNA recognition by the RAGE receptor. J Exp Med, 211, 749-750. PubMed id: 24778420 DOI: 10.1084/jem.20132526

Date:

15-Jan-14 Release date: 23-Apr-14

Protein chains

Q15109  (RAGE_HUMAN) -  Advanced glycosylation end product-specific receptor from Homo sapiens

Seq: 404 a.a.

Struc: 211 a.a.

DOI no: 10.1084/jem.20132526

J Exp Med 211:749-750 (2014)

PubMed id: 24778420

The specificity of DNA recognition by the RAGE receptor.

L.Yatime, G.R.Andersen.

ABSTRACT

A recent paper by Sirois et al. in The Journal of Experimental Medicine reports that the receptor for advanced glycation end-products (RAGE) promotes uptake of DNA into endosomes and lowers the immune recognition threshold for the activation of Toll-like receptor 9. Two crystal structures suggested that the DNA phosphate-deoxyribose backbone is recognized by RAGE through well-defined interactions. However, the electron densities for the DNA molecules are weak enough that the presented modeling of DNA is questionable, and models only containing RAGE account for the observed diffraction data just as well as the RAGE-DNA complexes presented by the authors.