UniProt functional annotation for P47709



	UniProt functional annotation for P47709

UniProt code: P47709.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Plays an essential role in docking and fusion steps of regulated exocytosis (PubMed:8617225, PubMed:16203731). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (PubMed:8617225). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability (PubMed:26679993). Plays also a role in the exocytosis of arginine vasopressin hormone (PubMed:29367474). {ECO:0000269|PubMed:16203731, ECO:0000269|PubMed:26679993, ECO:0000269|PubMed:29367474, ECO:0000269|PubMed:8617225}.

Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};

Subunit: Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B (By similarity). Interacts with RAB3A; this interaction recruits RPH3A to synaptic vesicules (PubMed:8617225, PubMed:10025402). Interacts (via C2B domain) with SNAP25 (PubMed:16203731, PubMed:28634303). Interacts with deubiquitinating enzyme CAND1; this interaction results in the deubiquitination of RPH3A (PubMed:29367474). Interacts with GRIN2A and DLG4; this ternary complex regulates NMDA receptor composition at postsynaptic membranes (PubMed:26679993). Interacts with SNCA (By similarity). {ECO:0000250|UniProtKB:P47708, ECO:0000250|UniProtKB:Q9Y2J0, ECO:0000269|PubMed:10025402, ECO:0000269|PubMed:16203731, ECO:0000269|PubMed:26679993, ECO:0000269|PubMed:28634303, ECO:0000269|PubMed:29367474, ECO:0000269|PubMed:8617225}.

Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269|PubMed:8060298, ECO:0000269|PubMed:8617225}. Cell projection, dendritic spine {ECO:0000269|PubMed:26679993}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000269|PubMed:26679993}. Membrane {ECO:0000269|PubMed:17166855}; Peripheral membrane protein {ECO:0000269|PubMed:17166855}.

Tissue specificity: Specifically expressed in brain.

Domain: Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers. {ECO:0000269|PubMed:17166855}.

Ptm: Ubiquitinated. Deubiquitinated by CAND1 to prevent its degradation. {ECO:0000269|PubMed:29367474}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Plays an essential role in docking and fusion steps of regulated exocytosis (PubMed:8617225, PubMed:16203731). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (PubMed:8617225). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability (PubMed:26679993). Plays also a role in the exocytosis of arginine vasopressin hormone (PubMed:29367474). {ECO:0000269\|PubMed:16203731, ECO:0000269\|PubMed:26679993, ECO:0000269\|PubMed:29367474, ECO:0000269\|PubMed:8617225}.


Cofactor:		Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255\|PROSITE-ProRule:PRU00041};
Subunit:		Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B (By similarity). Interacts with RAB3A; this interaction recruits RPH3A to synaptic vesicules (PubMed:8617225, PubMed:10025402). Interacts (via C2B domain) with SNAP25 (PubMed:16203731, PubMed:28634303). Interacts with deubiquitinating enzyme CAND1; this interaction results in the deubiquitination of RPH3A (PubMed:29367474). Interacts with GRIN2A and DLG4; this ternary complex regulates NMDA receptor composition at postsynaptic membranes (PubMed:26679993). Interacts with SNCA (By similarity). {ECO:0000250\|UniProtKB:P47708, ECO:0000250\|UniProtKB:Q9Y2J0, ECO:0000269\|PubMed:10025402, ECO:0000269\|PubMed:16203731, ECO:0000269\|PubMed:26679993, ECO:0000269\|PubMed:28634303, ECO:0000269\|PubMed:29367474, ECO:0000269\|PubMed:8617225}.
Subcellular location:		Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269\|PubMed:8060298, ECO:0000269\|PubMed:8617225}. Cell projection, dendritic spine {ECO:0000269\|PubMed:26679993}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000269\|PubMed:26679993}. Membrane {ECO:0000269\|PubMed:17166855}; Peripheral membrane protein {ECO:0000269\|PubMed:17166855}.
Tissue specificity:		Specifically expressed in brain.
Domain:		Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers. {ECO:0000269\|PubMed:17166855}.
Ptm:		Ubiquitinated. Deubiquitinated by CAND1 to prevent its degradation. {ECO:0000269\|PubMed:29367474}.