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PDBsum entry 4npk
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Membrane protein
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PDB id
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4npk
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References listed in PDB file
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Key reference
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Title
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Structure and ca²⁺-Binding properties of the tandem c₂ domains of e-Syt2.
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Authors
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J.Xu,
T.Bacaj,
A.Zhou,
D.R.Tomchick,
T.C.Südhof,
J.Rizo.
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Ref.
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Structure, 2014,
22,
269-280.
[DOI no: ]
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PubMed id
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Abstract
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Contacts between the endoplasmic reticulum and the plasma membrane involve
extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins
with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to
bind Ca(2+), but no Ca(2+)-dependent function has been attributed to this
protein. We have determined the crystal structures of the tandem C2 domains of
E-Syt2 in the absence and presence of Ca(2+) and analyzed their Ca(2+)-binding
properties by nuclear magnetic resonance spectroscopy. Our data reveal an
unexpected V-shaped structure with a rigid orientation between the two C2
domains that is not substantially altered by Ca(2+). The E-Syt2 C2A domain binds
up to four Ca(2+) ions, whereas the C2B domain does not bind Ca(2+). These
results suggest that E-Syt2 performs an as yet unidentified Ca(2+)-dependent
function through its C2A domain and uncover fundamental differences between the
properties of the tandem C2 domains of E-Syts and synaptotagmins.
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Secondary reference #1
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Title
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E-Syts, A family of membranous ca2+-Sensor proteins with multiple c2 domains.
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Authors
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S.W.Min,
W.P.Chang,
T.C.Südhof.
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Ref.
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Proc Natl Acad Sci U S A, 2007,
104,
3823-3828.
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PubMed id
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