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PDBsum entry 4npk
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Membrane protein
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PDB id
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4npk
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PDB id:
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| Name: |
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Membrane protein
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Title:
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Extended-synaptotagmin 2, c2a- and c2b-domains, calcium bound
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Structure:
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Extended synaptotagmin-2. Chain: a. Fragment: c2a and c2b domains. Synonym: e-syt2, chr2syt. Engineered: yes. Mutation: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: esyt2, extended-synaptotagmin 2, fam62b, kiaa1228. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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2.55Å
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R-factor:
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0.225
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R-free:
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0.286
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Authors:
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D.R.Tomchick,J.Rizo,J.Xu
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Key ref:
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J.Xu
et al.
(2014).
Structure and Ca²⁺-binding properties of the tandem C₂ domains of E-Syt2.
Structure,
22,
269-280.
PubMed id:
DOI:
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Date:
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21-Nov-13
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Release date:
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29-Jan-14
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PROCHECK
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Headers
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References
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A0FGR8
(ESYT2_HUMAN) -
Extended synaptotagmin-2 from Homo sapiens
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Seq:
Struc:
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921 a.a.
269 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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DOI no:
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Structure
22:269-280
(2014)
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PubMed id:
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Structure and Ca²⁺-binding properties of the tandem C₂ domains of E-Syt2.
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J.Xu,
T.Bacaj,
A.Zhou,
D.R.Tomchick,
T.C.Südhof,
J.Rizo.
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ABSTRACT
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Contacts between the endoplasmic reticulum and the plasma membrane involve
extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins
with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to
bind Ca(2+), but no Ca(2+)-dependent function has been attributed to this
protein. We have determined the crystal structures of the tandem C2 domains of
E-Syt2 in the absence and presence of Ca(2+) and analyzed their Ca(2+)-binding
properties by nuclear magnetic resonance spectroscopy. Our data reveal an
unexpected V-shaped structure with a rigid orientation between the two C2
domains that is not substantially altered by Ca(2+). The E-Syt2 C2A domain binds
up to four Ca(2+) ions, whereas the C2B domain does not bind Ca(2+). These
results suggest that E-Syt2 performs an as yet unidentified Ca(2+)-dependent
function through its C2A domain and uncover fundamental differences between the
properties of the tandem C2 domains of E-Syts and synaptotagmins.
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Links
