UniProt functional annotation for D1A4G7



	UniProt functional annotation for D1A4G7

UniProt code: D1A4G7.

Organism: Thermomonospora curvata (strain ATCC 19995 / DSM 43183 / JCM 3096 / KCTC 9072 / NBRC 15933 / NCIMB 10081 / Henssen B9).

Taxonomy: Bacteria; Actinobacteria; Streptosporangiales; Thermomonosporaceae; Thermomonospora.

Function: Part of the ESX specialized secretion system, which exports proteins from the cell including EsxA (ESAT-6) and EsxB (CFP-10) (PubMed:25865481). Has weak intrinsic ATPase activity; probably only the first FtsK domain can hydrolyze ATP (PubMed:25865481). Might be the translocase subunit (PubMed:25865481). {ECO:0000269|PubMed:25865481}.

Activity regulation: EsxB binding to the third FtsK domain causes multimerization; a subsequent unknown step relieves the allosteric inhibition of linker 2 on FtsK domain 1, activating the ATPase activity; a mutant EsxB ('Ala-98') does not cause multimers to form (PubMed:25865481). {ECO:0000269|PubMed:25865481}.

Subunit: The cytosolic domain can form homodimers (PubMed:25865481). Binds EsxB, which leads to multimerization, however EsxA disassembles the multimers, possibly by making EccC-EsxA-EsxB trimers instead of EccC-EsxB-EsxB-EccC tetramers. Forms a complex with EsxA and EsxB, probably wholly mediated by EsxB (PubMed:25865481). {ECO:0000269|PubMed:25865481}.

Subcellular location: Cell membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.

Domain: The cytoplasmic domain is a rigid structure with 4 domains (residues 276 to 401 form an unnamed domain) plus the 3 FtsK (ATPase) domains which are connected by short linkers. Binds EsxB via a small pocket (residues 1163-1208) in the third FtsK (ATPase) domain; the linkers between FtsK 1-2 and FtsK 2-3 bind in an analogous manner to FtsK 1 and FtsK 2. Linker 2 binding to FtsK 1 decreases its ATPase activity and probably controls it (PubMed:25865481). {ECO:0000269|PubMed:25865481}.

Miscellaneous: Unlike the well characterized M.tuberculosis ESX-1 cluster, this protein is not split into 2 genes. This subunit and a WGX100 family protein are the only proteins universally associated with T7SS. {ECO:0000305|PubMed:25865481}.

Annotations taken from UniProtKB at the EBI.


Organism:		Thermomonospora curvata (strain ATCC 19995 / DSM 43183 / JCM 3096 / KCTC 9072 / NBRC 15933 / NCIMB 10081 / Henssen B9).
Taxonomy:		Bacteria; Actinobacteria; Streptosporangiales; Thermomonosporaceae; Thermomonospora.



Function:		Part of the ESX specialized secretion system, which exports proteins from the cell including EsxA (ESAT-6) and EsxB (CFP-10) (PubMed:25865481). Has weak intrinsic ATPase activity; probably only the first FtsK domain can hydrolyze ATP (PubMed:25865481). Might be the translocase subunit (PubMed:25865481). {ECO:0000269\|PubMed:25865481}.


Activity regulation:		EsxB binding to the third FtsK domain causes multimerization; a subsequent unknown step relieves the allosteric inhibition of linker 2 on FtsK domain 1, activating the ATPase activity; a mutant EsxB ('Ala-98') does not cause multimers to form (PubMed:25865481). {ECO:0000269\|PubMed:25865481}.
Subunit:		The cytosolic domain can form homodimers (PubMed:25865481). Binds EsxB, which leads to multimerization, however EsxA disassembles the multimers, possibly by making EccC-EsxA-EsxB trimers instead of EccC-EsxB-EsxB-EccC tetramers. Forms a complex with EsxA and EsxB, probably wholly mediated by EsxB (PubMed:25865481). {ECO:0000269\|PubMed:25865481}.
Subcellular location:		Cell membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.
Domain:		The cytoplasmic domain is a rigid structure with 4 domains (residues 276 to 401 form an unnamed domain) plus the 3 FtsK (ATPase) domains which are connected by short linkers. Binds EsxB via a small pocket (residues 1163-1208) in the third FtsK (ATPase) domain; the linkers between FtsK 1-2 and FtsK 2-3 bind in an analogous manner to FtsK 1 and FtsK 2. Linker 2 binding to FtsK 1 decreases its ATPase activity and probably controls it (PubMed:25865481). {ECO:0000269\|PubMed:25865481}.
Miscellaneous:		Unlike the well characterized M.tuberculosis ESX-1 cluster, this protein is not split into 2 genes. This subunit and a WGX100 family protein are the only proteins universally associated with T7SS. {ECO:0000305\|PubMed:25865481}.