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PDBsum entry 4n8v
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Immune system
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PDB id
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4n8v
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Contents |
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190 a.a.
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274 a.a.
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100 a.a.
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References listed in PDB file
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Key reference
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Title
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Activating killer cell immunoglobulin-Like receptor 2ds2 binds to hla-A11.
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Authors
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J.Liu,
Z.Xiao,
H.L.Ko,
M.Shen,
E.C.Ren.
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Ref.
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Proc Natl Acad Sci U S A, 2014,
111,
2662-2667.
[DOI no: ]
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PubMed id
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Abstract
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Inhibitory killer cell Ig-like receptors (KIRs) are known to recognize HLA
ligands mainly of the HLA-C and Bw4 groups, but the ligands for KIRs are poorly
understood. We report here the identification of the cognate ligand for the
activating KIR 2DS2 as HLA-A*11:01. The crystal structure of the
KIR2DS2-HLA-A*11:01 complex was solved at 2.5-Å resolution and revealed
residue-binding characteristics distinct from those of inhibitory KIRs with
HLA-C and the critical role of residues Tyr45 and Asp72 in shaping binding
specificity to HLA-A*11:01. Using KIR2DS2 tetramers, binding to surface
HLA-A*11:01 on live cells was demonstrated and, furthermore, that binding can be
altered by residue changes at p8 of the peptide, indicating the influence of
peptide sequence on KIR-HLA association. In addition, heteronuclear single
quantum coherence NMR was used to map the involvement of critical residues in
HLA binding at the interface of KIR and HLA, and validates the data observed in
the crystal structure. Our data provide structural evidence of the recognition
of A*11:01 by the activating KIR2DS2 and extend our understanding of the KIR-HLA
binding spectrum.
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