PDBsum entry 4n78

Protein binding

PDB id: 4n78

Contents

Protein chains

1184 a.a.

1085 a.a.

202 a.a.

67 a.a.

156 a.a.

Ligands

ARG-SER-MET-SER-THR-PHE-GLY-LYS

GOL ×16

Metals

_CL ×4

Waters ×746

PDB id:

4n78

Name:

Protein binding

Title:

The wave regulatory complex links diverse receptors to the actin cytoskeleton

Structure:

Cytoplasmic fmr1-interacting protein 1. Chain: a. Synonym: specifically rac1-associated protein 1, sra-1, p140sra-1. Engineered: yes. Nck-associated protein 1. Chain: b. Synonym: nap 1, membrane-associated protein hem-2, p125nap1. Engineered: yes. Wiskott-aldrich syndrome protein family member 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cyfip1, kiaa0068. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: nckap1, hem2, kiaa0587, nap1. Gene: wasf1, kiaa0269, scar1, wave1. Expressed in: escherichia coli.

Resolution:

2.43Å R-factor: 0.189 R-free: 0.209

Authors:

Z.C.Chen

Key ref:

B.Chen et al. (2014). The WAVE regulatory complex links diverse receptors to the actin cytoskeleton. Cell, 156, 195-207. PubMed id: 24439376 DOI: 10.1016/j.cell.2013.11.048

Date:

15-Oct-13 Release date: 05-Feb-14

Protein chain

Q7L576  (CYFP1_HUMAN) -  Cytoplasmic FMR1-interacting protein 1 from Homo sapiens

Seq: 1253 a.a.

Struc: 1184 a.a.

Protein chain

Q9Y2A7  (NCKP1_HUMAN) -  Nck-associated protein 1 from Homo sapiens

Seq: 1128 a.a.

Struc: 1085 a.a.

Protein chain

Q92558  (WASF1_HUMAN) -  Actin-binding protein WASF1 from Homo sapiens

Seq: 559 a.a.

Struc: 202 a.a.*

Protein chain

Q8WUW1  (BRK1_HUMAN) -  Protein BRICK1 from Homo sapiens

Seq: 75 a.a.

Struc: 67 a.a.

Protein chain

Q9NYB9  (ABI2_HUMAN) -  Abl interactor 2 from Homo sapiens

Seq: 513 a.a.

Struc: 156 a.a.

* PDB and UniProt seqs differ at 36 residue positions (black crosses)

DOI no: 10.1016/j.cell.2013.11.048

Cell 156:195-207 (2014)

PubMed id: 24439376

The WAVE regulatory complex links diverse receptors to the actin cytoskeleton.

B.Chen, K.Brinkmann, Z.Chen, C.W.Pak, Y.Liao, S.Shi, L.Henry, N.V.Grishin, S.Bogdan, M.K.Rosen.

ABSTRACT

The WAVE regulatory complex (WRC) controls actin cytoskeletal dynamics throughout the cell by stimulating the actin-nucleating activity of the Arp2/3 complex at distinct membrane sites. However, the factors that recruit the WRC to specific locations remain poorly understood. Here, we have identified a large family of potential WRC ligands, consisting of ∼120 diverse membrane proteins, including protocadherins, ROBOs, netrin receptors, neuroligins, GPCRs, and channels. Structural, biochemical, and cellular studies reveal that a sequence motif that defines these ligands binds to a highly conserved interaction surface of the WRC formed by the Sra and Abi subunits. Mutating this binding surface in flies resulted in defects in actin cytoskeletal organization and egg morphology during oogenesis, leading to female sterility. Our findings directly link diverse membrane proteins to the WRC and actin cytoskeleton and have broad physiological and pathological ramifications in metazoans.