PDBsum entry 4mz3

Membrane protein

PDB id: 4mz3

Contents

Protein chains

120 a.a.

Waters ×272

References listed in PDB file

Key reference

• Title: Crystallographic insights into sodium-Channel modulation by the β4 subunit.
• Authors: J.Gilchrist, S.Das, F.Van petegem, F.Bosmans.
• Ref.: Proc Natl Acad Sci U S A, 2013, 110, E5016. [DOI no: 10.1073/pnas.1314557110]
• PubMed id: 24297919

Abstract

Voltage-gated sodium (Nav) channels are embedded in a multicomponent membrane signaling complex that plays a crucial role in cellular excitability. Although the mechanism remains unclear, β-subunits modify Nav channel function and cause debilitating disorders when mutated. While investigating whether β-subunits also influence ligand interactions, we found that β4 dramatically alters toxin binding to Nav1.2. To explore these observations further, we solved the crystal structure of the extracellular β4 domain and identified (58)Cys as an exposed residue that, when mutated, eliminates the influence of β4 on toxin pharmacology. Moreover, our results suggest the presence of a docking site that is maintained by a cysteine bridge buried within the hydrophobic core of β4. Disrupting this bridge by introducing a β1 mutation implicated in epilepsy repositions the (58)Cys-containing loop and disrupts β4 modulation of Nav1.2. Overall, the principles emerging from this work (i) help explain tissue-dependent variations in Nav channel pharmacology; (ii) enable the mechanistic interpretation of β-subunit-related disorders; and (iii) provide insights in designing molecules capable of correcting aberrant β-subunit behavior.