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UniProt functional annotation for P40416 | ||
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| UniProt code: P40416. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins (PubMed:10406803, PubMed:31040179, PubMed:25006243). Hydrolyzes ATP (PubMed:25006243, PubMed:24604199). Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound (PubMed:24604199, PubMed:25006243, PubMed:31040179). {ECO:0000269|PubMed:10406803, ECO:0000269|PubMed:24604199, ECO:0000269|PubMed:25006243, ECO:0000269|PubMed:31040179}. |
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| Biophysicochemical properties: | |
Kinetic parameters: KM=109 uM for glutathione disulfide {ECO:0000269|PubMed:25006243}; Vmax=27.4 pmol/min/mg enzyme {ECO:0000269|PubMed:25006243}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:24604199}. |
| Subcellular location: | |
Mitochondrion inner membrane {ECO:0000269|PubMed:24604199, ECO:0000269|PubMed:7828591}; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:24604199, ECO:0000269|PubMed:25006243, ECO:0000269|PubMed:7828591}. |
| Disruption phenotype: | |
Impairs cytosolic iron-sulfur (Fe-S) cluster assembly and decreases cytosolic tRNA thiolation. {ECO:0000269|PubMed:31040179}. |
| Miscellaneous: | |
Present with 3250 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.