PDBsum entry 4mrx

Luminescent protein

PDB id: 4mrx

Contents

Protein chain

189 a.a.

Ligands

CZH

Waters ×298

PDB id:

4mrx

Name:

Luminescent protein

Title:

Crystal structure of y138f obelin mutant from obelia longissima at 1.72 angstrom resolution

Structure:

Obelin. Chain: a. Synonym: obl. Engineered: yes. Mutation: yes

Source:

Obelia longissima. Organism_taxid: 32570. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.72Å R-factor: 0.142 R-free: 0.169

Authors:

P.V.Natashin,W.Ding,E.V.Eremeeva,S.V.Markova,J.Lee,E.S.Vysotski, Z.J.Liu

Key ref:

P.V.Natashin et al. (2014). Structures of the Ca2+-regulated photoprotein obelin Y138F mutant before and after bioluminescence support the catalytic function of a water molecule in the reaction. Acta Crystallogr D Biol Crystallogr, 70, 720-732. PubMed id: 24598741 DOI: 10.1107/S1399004713032434

Date:

17-Sep-13 Release date: 12-Mar-14

Protein chain

Q27709  (OBL_OBELO) -  Obelin from Obelia longissima

Seq: 195 a.a.

Struc: 189 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1107/S1399004713032434

Acta Crystallogr D Biol Crystallogr 70:720-732 (2014)

PubMed id: 24598741

Structures of the Ca2+-regulated photoprotein obelin Y138F mutant before and after bioluminescence support the catalytic function of a water molecule in the reaction.

P.V.Natashin, W.Ding, E.V.Eremeeva, S.V.Markova, J.Lee, E.S.Vysotski, Z.J.Liu.

ABSTRACT

Ca(2+)-regulated photoproteins, which are responsible for light emission in a variety of marine coelenterates, are a highly valuable tool for measuring Ca(2+) inside living cells. All of the photoproteins are a single-chain polypeptide to which a 2-hydroperoxycoelenterazine molecule is tightly but noncovalently bound. Bioluminescence results from the oxidative decarboxylation of 2-hydroperoxycoelenterazine, generating protein-bound coelenteramide in an excited state. Here, the crystal structures of the Y138F obelin mutant before and after bioluminescence are reported at 1.72 and 1.30 Å resolution, respectively. The comparison of the spatial structures of the conformational states of Y138F obelin with those of wild-type obelin gives clear evidence that the substitution of Tyr by Phe does not affect the overall structure of both Y138F obelin and its product following Ca(2+) discharge compared with the corresponding conformational states of wild-type obelin. Despite the similarity of the overall structures and internal cavities of Y138F and wild-type obelins, there is a substantial difference: in the cavity of Y138F obelin a water molecule corresponding to W2 in wild-type obelin is not found. However, in Ca(2+)-discharged Y138F obelin this water molecule now appears in the same location. This finding, together with the observed much slower kinetics of Y138F obelin, clearly supports the hypothesis that the function of a water molecule in this location is to catalyze the 2-hydroperoxycoelenterazine decarboxylation reaction by protonation of a dioxetanone anion before its decomposition into the excited-state product. Although obelin differs from other hydromedusan Ca(2+)-regulated photoproteins in some of its properties, they are believed to share a common mechanism.